Controlling the spatial organization of cells and extracellular matrix proteins in engineered tissues using ultrasound standing wave fields.
Controlling the spatial organization of cells and extracellular matrix proteins in engineered tissues using ultrasound standing wave fields.
复制标题
DOI：
10.1016/j.ultrasmedbio.2010.08.007
复制
发表时间：
2010-11
影响因子：
2.9
通讯作者：
Dalecki, Diane
中科院分区：
文献类型：
--
作者：
Garvin, Kelley A.;Hocking, Denise C.;Dalecki, Diane
关键词：
组织工程学在挽救每年在等待器官捐献过程中死亡的数千名器官移植患者的生命方面具有巨大的潜力。然而，为了成功地在体外构建组织和器官，需要重建适当的细胞外微环境来促进组织再生的方法。在这项研究中，我们开发了超声驻波场技术在组织工程领域的应用。超声辐射力被用来非侵入性地控制哺乳动物细胞和细胞结合的细胞外基质蛋白在三维胶原基工程组织中的空间分布。细胞悬浮在未聚合的胶原溶液中，并在室温下暴露于1 MHz源产生的连续波USWF 15分钟。在USWF照射过程中，胶原蛋白发生聚合，形成具有明显聚集细胞条带的三维胶原凝胶。细胞带的密度取决于初始细胞浓度和USWF的压力幅值。重要的是，USWF暴露并没有降低细胞存活率，而是增强了细胞功能。与细胞分布均匀的假暴露样本相比，将细胞排列成松散聚集的平面细胞带显著增加了细胞介导的胶原凝胶收缩和胶原纤维重组的水平。此外，在USWF暴露前，通过将细胞外基质蛋白FN结合到细胞表面，将其定位于细胞带状区域。通过控制细胞和细胞外的组织，USWF技术的应用是一种很有前途的体外组织工程方法。
Tissue engineering holds great potential for saving the lives of thousands of organ transplant patients who die each year while waiting for donor organs. However, to successfully fabricate tissues and organs in vitro, methodologies that recreate appropriate extracellular microenvironments to promote tissue regeneration are needed. In this study, we have developed an application of ultrasound standing wave field (USWF) technology to the field of tissue engineering. Acoustic radiation forces associated with USWF were used to non-invasively control the spatial distribution of mammalian cells and cell-bound extracellular matrix proteins within three-dimensional collagen-based engineered tissues. Cells were suspended in unpolymerized collagen solutions and were exposed to a continuous wave USWF, generated using a 1 MHz source, for 15 min at room temperature. Collagen polymerization occurred during USWF exposure resulting in the formation of three-dimensional collagen gels with distinct bands of aggregated cells. The density of cell bands was dependent on both the initial cell concentration and the pressure amplitude of the USWF. Importantly, USWF exposure did not decrease cell viability, but rather enhanced cell function. Alignment of cells into loosely clustered, planar cell bands significantly increased levels of cell-mediated collagen gel contraction and collagen fiber reorganization as compared to sham-exposed samples with a homogeneous cell distribution. Additionally, the extracellular matrix protein, fibronectin, was localized to cell banded areas by binding the protein to the cell surface prior to USWF exposure. By controlling cell and extracellular organization, this application of USWF technology is a promising approach for engineering tissues in vitro.
登录
查看更多内容
影响因子：
2.4
作者：
Baddour, RE;Sherar, MD;Kolios, MC
通讯作者：
Kolios, MC
影响因子：
8
作者：
Fischer, H;Polikarpov, I;Craievich, AF
通讯作者：
Craievich, AF
影响因子：
17.3
作者：
Coakley, WT
通讯作者：
Coakley, WT
影响因子：
3.6
作者：
FREUND, I;DEUTSCH, M
通讯作者：
DEUTSCH, M
影响因子：
2.9
作者：
Hultstrom, J.;Manneberg, O.;Wiklund, M.
通讯作者：
Wiklund, M.