Potential Direct Regulators of the Drosophila yellow Gene Identified by Yeast One-Hybrid and RNAi Screens.
Potential Direct Regulators of the Drosophila yellow Gene Identified by Yeast One-Hybrid and RNAi Screens.
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酵母 One-Hybrid 和 RNAi 筛选鉴定出果蝇黄色基因的潜在直接调节因子。
DOI：
10.1534/g3.116.032607
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发表时间：
2016-10-13
期刊：
影响因子：
--
通讯作者：
Wittkopp PJ
中科院分区：
文献类型：
--
作者：
Kalay G;Lusk R;Dome M;Hens K;Deplancke B;Wittkopp PJ
基因表达的调控控制着发育，而这种调控的变化往往有助于表型进化。果蝇色素沉着是研究基因调控进化变化的模型系统，与色素模式不同相关的黄色等色素沉着基因在不同物种间的表达差异被证明是由顺式和反式调控的变化引起的。目前，人们对发散性黄色表达的顺式调控成分比反式调控成分了解得更多，部分原因是很少有黄色表达的反式调控被确定。本研究旨在通过结合酵母单杂交和RNAi筛选分别结合黄色顺式调控序列和影响成人腹部色素沉著的转录因子，提高我们对黄色表达的反式控制的理解。在酵母-单杂交筛选的670个转录因子中，有45个显示出与D. melanogaster、D. pseudoobscura和D. willistoni的5 '基因间和内含子黄色序列片段结合的证据，这表明它们可能是黄色表达的直接调节剂。在酵母-一杂交筛选的670个转录因子中，加上另一个先前被证明是黄色基因上游的TF， 125个也使用RNAi进行了测试，32个显示腹部色素沉着改变。在两种筛选中均鉴定出9个转录因子，包括4个与蜕皮激素信号相关的核受体（Hr78、Hr38、Hr46和Eip78C）。这一发现提示，在成虫色素形成的早期蛹发育过程中，黄色的表达可能直接受到受蜕皮激素影响的核受体的控制。
The regulation of gene expression controls development, and changes in this regulation often contribute to phenotypic evolution. Drosophila pigmentation is a model system for studying evolutionary changes in gene regulation, with differences in expression of pigmentation genes such as yellow that correlate with divergent pigment patterns among species shown to be caused by changes in cis- and trans-regulation. Currently, much more is known about the cis-regulatory component of divergent yellow expression than the trans-regulatory component, in part because very few trans-acting regulators of yellow expression have been identified. This study aims to improve our understanding of the trans-acting control of yellow expression by combining yeast-one-hybrid and RNAi screens for transcription factors binding to yellow cis-regulatory sequences and affecting abdominal pigmentation in adults, respectively. Of the 670 transcription factors included in the yeast-one-hybrid screen, 45 showed evidence of binding to one or more sequence fragments tested from the 5′ intergenic and intronic yellow sequences from D. melanogaster, D. pseudoobscura, and D. willistoni, suggesting that they might be direct regulators of yellow expression. Of the 670 transcription factors included in the yeast-one-hybrid screen, plus another TF previously shown to be genetically upstream of yellow, 125 were also tested using RNAi, and 32 showed altered abdominal pigmentation. Nine transcription factors were identified in both screens, including four nuclear receptors related to ecdysone signaling (Hr78, Hr38, Hr46, and Eip78C). This finding suggests that yellow expression might be directly controlled by nuclear receptors influenced by ecdysone during early pupal development when adult pigmentation is forming.
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影响因子：
64.5
作者：
Jeong, Sangyun;Rokas, Antonis;Carroll, Sean B.
通讯作者：
Carroll, Sean B.
影响因子：
11.4
作者：
CELNIKER, SE;SHARMA, S;LEWIS, EB
通讯作者：
LEWIS, EB
影响因子：
10.5
作者：
GEYER, PK;CORCES, VG
通讯作者：
CORCES, VG
影响因子：
4.5
作者：
Danielsen ET;Moeller ME;Dorry E;Komura-Kawa T;Fujimoto Y;Troelsen JT;Herder R;O'Connor MB;Niwa R;Rewitz KF
通讯作者：
Rewitz KF
影响因子：
7
作者：
Deplancke, B;Dupuy, D;Walhout, AJM
通讯作者：
Walhout, AJM