Draft genome sequence and characterization of commensal Escherichia coli strain BG1 isolated from bovine gastro-intestinal tract.
Draft genome sequence and characterization of commensal Escherichia coli strain BG1 isolated from bovine gastro-intestinal tract.
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DOI：
10.1186/s40793-017-0272-0
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发表时间：
2017
影响因子：
--
通讯作者：
Forano E
中科院分区：
文献类型：
--
作者：
Segura A;Auffret P;Klopp C;Bertin Y;Forano E
大肠杆菌是哺乳动物胃肠道中最丰富的兼性厌氧细菌，但由于获得毒力因子，可能导致肠道感染。致病性大肠杆菌菌株的基因组已被广泛描述，而牛共生大肠杆菌菌株的基因组却非常稀少。在这里，我们报告了从牛胃肠道分离的共生大肠杆菌 BG1 的基因组序列、注释和特征。全基因组测序分析显示，BG1 的染色体长 4,782,107 bp，编码 4465 个蛋白质和 97 个 RNA。大肠杆菌BG1属于血清型O159:H21，属于B1系统群，拥有编码“毒力因子”的遗传信息，例如粘附系统、铁获取和鞭毛合成。总共检测到 12 个粘附系统，反映了 BG1 定植牛胃肠道不同部分的潜在能力。大肠杆菌 BG1 无法同化乙醇胺，而乙醇胺为牛胃肠道中的某些致病性大肠杆菌提供了营养优势。基因组分析揭示了 i) 由于编码乙醇胺转运和同化的基因中的非同义 SNP 导致 34 个氨基酸发生变化，以及 ii) 插入钴胺素腺苷转移酶（乙醇胺同化所需的关键酶）中的额外预测的 α 螺旋。这些修饰可以解释 BG1 无法使用乙醇胺的原因。 BG1基因组现在可以作为后续进化和比较研究的参考（对照菌株）。本文的在线版本 (10.1186/s40793-017-0272-0) 包含补充材料，可供授权用户使用。
Escherichia coli is the most abundant facultative anaerobic bacteria in the gastro-intestinal tract of mammals but can be responsible for intestinal infection due to acquisition of virulence factors. Genomes of pathogenic E. coli strains are widely described whereas those of bovine commensal E. coli strains are very scarce. Here, we report the genome sequence, annotation, and features of the commensal E. coli BG1 isolated from the gastro-intestinal tract of cattle. Whole genome sequencing analysis showed that BG1 has a chromosome of 4,782,107 bp coding for 4465 proteins and 97 RNAs. E. coli BG1 belonged to the serotype O159:H21, was classified in the phylogroup B1 and possessed the genetic information encoding “virulence factors” such as adherence systems, iron acquisition and flagella synthesis. A total of 12 adherence systems were detected reflecting the potential ability of BG1 to colonize different segments of the bovine gastro-intestinal tract. E. coli BG1 is unable to assimilate ethanolamine that confers a nutritional advantage to some pathogenic E. coli in the bovine gastro-intestinal tract. Genome analysis revealed the presence of i) 34 amino acids change due to non-synonymous SNPs among the genes encoding ethanolamine transport and assimilation, and ii) an additional predicted alpha helix inserted in cobalamin adenosyltransferase, a key enzyme required for ethanolamine assimilation. These modifications could explain the incapacity of BG1 to use ethanolamine. The BG1 genome can now be used as a reference (control strain) for subsequent evolution and comparative studies. The online version of this article (10.1186/s40793-017-0272-0) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
通讯作者：
Pourcel, Christine
影响因子：
14.9
作者：
Geer LY;Marchler-Bauer A;Geer RC;Han L;He J;He S;Liu C;Shi W;Bryant SH
通讯作者：
Bryant SH
影响因子：
5.1
作者：
Bertin, Yolande;Girardeau, J. P.;Martin, Christine
通讯作者：
Martin, Christine
DOI：
10.3390/ijerph120100970
发表时间：
2015-01-01
影响因子：
--
作者：
Bok, Ewa;Mazurek, Justyna;Baldy-Chudzik, Katarzyna
通讯作者：
Baldy-Chudzik, Katarzyna
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.