Genomic characterization of carbapenem-non-susceptible Pseudomonas aeruginosa in Singapore.
Genomic characterization of carbapenem-non-susceptible Pseudomonas aeruginosa in Singapore.
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DOI：
10.1080/22221751.2021.1968318
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发表时间：
2021-12
影响因子：
13.2
通讯作者：
Ong RT
中科院分区：
文献类型：
--
作者：
Teo JQ;Tang CY;Lim JC;Lee SJ;Tan SH;Koh TH;Sim JH;Tan TT;Kwa AL;Ong RT
铜绿假单胞菌是临床上重要的病原体，涉及许多医院获得性感染。其获得广谱耐药性的倾向使其成为一种严重的公共卫生威胁，需要采取紧急控制措施。虽然基于全基因组测序的基因组监测提供了一种追踪抗菌素耐药性的手段，但其在铜绿假单胞菌分子流行病学调查中的应用仍然有限，特别是在东南亚地区。我们对2006-2020年在新加坡最大的公立急症医院收集的222株碳青霉烯类非敏感铜绿假单胞菌（CNPA）的全基因组进行了测序。采用微量肉汤稀释法测定药敏。克隆亲缘性、多位点序列类型和耐药性决定因素（获得性和染色体性）被确定。在本研究中，CNPA表现出广谱耐药（87.8%），仅对多粘菌素B（95.0%）和阿米卡星（55.0%）保持敏感性。51.4%的分离菌检出碳青霉烯酶，其中以IMP和NDM金属β-内酰胺酶最为常见。碳青霉烯耐药也可能与OprD改变或外排机制（ArmZ/NalD突变）有关，这发生在有无碳青霉烯酶的菌株中。该医院的CNPA人群是多样化的；222株分离物可分为68种序列型（ST），其中包括各种高危克隆。我们发现了一个新兴的克隆，产生ndm -1的ST308，除了全球高风险的ST235克隆，这是我们群体中的优势克隆。因此，我们的研究结果提供了CNPA在当地循环谱系的“快照”，以及导致碳青霉烯类耐药性的普遍遗传机制。该数据库也可作为未来前瞻性监测研究的基线。
Pseudomonas aeruginosa is a clinically important pathogen implicated in many hospital-acquired infections. Its propensity to acquire broad-spectrum resistance has earned the organism its status as a severe public health threat requiring urgent control measures. While whole-genome sequencing-based genomic surveillance provides a means to track antimicrobial resistance, its use in molecular epidemiological surveys of P. aeruginosa remains limited, especially in the Southeast Asian region. We sequenced the whole genomes of 222 carbapenem-non-susceptible P. aeruginosa (CNPA) isolates collected in 2006–2020 at the largest public acute care hospital in Singapore. Antimicrobial susceptibilities were determined using broth microdilution. Clonal relatedness, multi-locus sequence types, and antimicrobial resistance determinants (acquired and chromosomal) were determined. In this study, CNPA exhibited broad-spectrum resistance (87.8% multi-drug resistance), retaining susceptibility only to polymyxin B (95.0%) and amikacin (55.0%). Carbapenemases were detected in 51.4% of the isolates, where IMP and NDM metallo-β-lactamases were the most frequent. Carbapenem resistance was also likely associated with OprD alterations or efflux mechanisms (ArmZ/NalD mutations), which occurred in strains with or without carbapenemases. The population of CNPA in the hospital was diverse; the 222 isolates grouped into 68 sequence types (ST), which included various high-risk clones. We detected an emerging clone, the NDM-1-producing ST308, in addition to the global high-risk ST235 clone which was the predominant clone in our population. Our results thus provide a “snapshot” of the circulating lineages of CNPA locally and the prevailing genetic mechanisms contributing to carbapenem resistance. This database also serves as the baseline for future prospective surveillance studies.
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DOI：
10.3390/antibiotics9100699
发表时间：
2020-10-15
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Hammer-Dedet F;Jumas-Bilak E;Licznar-Fajardo P
通讯作者：
Licznar-Fajardo P
影响因子：
9.4
作者：
Koh, TH;Wang, GCY;Sng, LH
通讯作者：
Sng, LH
影响因子：
4.9
作者：
Chen, Zhihui;Xu, Ziqin;Chen, Yangfang
通讯作者：
Chen, Yangfang
影响因子：
5.2
作者：
Juan, Carlos;Zamorano, Laura;Oliver, Antonio
通讯作者：
Oliver, Antonio
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.