Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy.
Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy.
复制标题
使用纳米喷雾解动电动质谱法与剪切力显微镜耦合到高分辨率组织成像。
DOI：
10.1007/s13361-017-1750-8
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发表时间：
2018-03
影响因子：
3.2
通讯作者：
Laskin J
中科院分区：
文献类型：
--
作者：
Nguyen SN;Sontag RL;Carson JP;Corley RA;Ansong C;Laskin J
将剪切力显微镜与纳米喷雾解吸电喷雾电离（nano-DESI）相结合，对组织切片进行了横向分辨率优于10 μm的恒定模式环境质谱成像（MSI）。剪切力显微镜能够精确控制MSI实验期间样品和纳米DESI探针之间的距离，并提供关于样品形貌的信息。使用分别代表海绵组织和致密组织的肺和脑组织切片进行概念验证实验。使用剪切力显微镜获得的形貌图像与在组织切片的相同区域上使用接触轮廓测定法获得的结果相当。发现组织高度的变化取决于组织类型，肺组织的范围为0-5 μm，脑组织切片的范围为0-3 μm。磷脂的离子图像与文献数据吻合较好。将纳米-DESI MSI图像归一化为加入到提取溶剂中的内标物的信号，使我们能够构建无基质效应的高分辨率离子图像。
Constant mode ambient mass spectrometry imaging (MSI) of tissue sections with high lateral resolution of better than 10 μm was performed by combining shear force microscopy with nanospray desorption electrospray ionization (nano-DESI). Shear force microscopy enabled precise control of the distance between the sample and nano-DESI probe during MSI experiments and provided information on sample topography. Proof-of-concept experiments were performed using lung and brain tissue sections representing spongy and dense tissues, respectively. Topography images obtained using shear force microscopy were comparable to the results obtained using contact profilometry over the same region of the tissue section. Variations in tissue height were found to be dependent on the tissue type and were in the range of 0–5 μm for lung tissue and 0–3 μm for brain tissue sections. Ion images of phospholipids obtained in this study are in good agreement with literature data. Normalization of nano-DESI MSI images to the signal of the internal standard added to the extraction solvent allowed us to construct high-resolution ion images free of matrix effects.
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影响因子：
2.3
作者：
Parson, Whitney B.;Koeniger, Stormy L.;Van Berkel, Gary J.
通讯作者：
Van Berkel, Gary J.
影响因子：
4.2
作者：
Roach, Patrick J.;Laskin, Julia;Laskin, Alexander
通讯作者：
Laskin, Alexander
影响因子：
7.4
作者：
Pan, Ning;Rao, Wei;Yang, Zhibo
通讯作者：
Yang, Zhibo
影响因子：
7.4
作者：
Laskin J;Lanekoff I
通讯作者：
Lanekoff I
影响因子：
2.3
作者：
Roempp, Andreas;Spengler, Bernhard
通讯作者：
Spengler, Bernhard