miR-146a-5p promotes replication of infectious bronchitis virus by targeting IRAK2 and TNFRSF18.
miR-146a-5p promotes replication of infectious bronchitis virus by targeting IRAK2 and TNFRSF18.
复制标题
DOI：
10.1016/j.micpath.2018.04.046
复制
发表时间：
2018-07
影响因子：
3.8
通讯作者：
Wang HN
中科院分区：
文献类型：
--
作者：
Liu H;Yang X;Zhang ZK;Zou WC;Wang HN
Avian infectious bronchitis virus (IBV) is a coronavirus which infects chickens (Gallus gallus) of all ages and causes significant economic losses to the poultry industry worldwide. The present study aims to analyze the miRNAs related to pathogenicity of nephropathogenic IBVs. It was found that four miRNAs (miR-1454, miR-3538, miR-146a-5p and miR-215-5p) were related to the infection of virulent nephropathogenic IBV with transcript per million (TPM) > 500 and more than a 2-fold alteration. In vitro study results showed that the alterations of these four miRNAs were consistent with in vivo data. In vitro, we found that high levels of miR-146a-5p could enhance the replication of IBV at the early stage of infection, and its down regulated level could slow down the replication of IBV. Finally, high levels of exogenous miR-146a-5p in HD11 cells led to down regulation of IL-1 receptor associated kinase-2 (IRAK2) and Tumor necrosis factor receptor superfamily member 18 (TNFRSF18) genes. Luciferase reporter assays revealed that miR-146a-5p could bind to the 3′-UTRs of IRAK2 and TNFRSF18. This is the first study demonstrating that IBV induced miR-146a-5p is related to virus pathogenesis by down regulating IRAK2 and TNFRSF18, which may serve as a therapeutic strategy for the prevention of IBV infections. It is proved that miR-146a-5p regulates the replication of IBV Beaudette strain in HD11 cells. The IRAK2 and TNFRSF18 genes in HD11 cells is the targeting inhibition by miR-146a-5p. The alterations of miR-1454, miR-3538, miR-146a-5p and miR-215-5p in HD11 cells were consistent with in vivo data.
登录
查看更多内容
影响因子：
13.6
作者：
Trobaugh DW;Klimstra WB
通讯作者：
Klimstra WB
影响因子：
8
作者：
Punj V;Matta H;Schamus S;Tamewitz A;Anyang B;Chaudhary PM
通讯作者：
Chaudhary PM
影响因子：
1.6
作者：
Fan, Wen-qiao;Wang, Hong-ning;Yang, Xin
通讯作者：
Yang, Xin
影响因子：
9.4
作者：
Bayry, J;Goudar, MS;Kolte, GN
通讯作者：
Kolte, GN
鸡肾响应不同烈性传染性支气管炎病毒感染的 MicroRNA 转录组分析
影响因子：
2.7
作者：
Yang, Xin;Gao, Wenqian;Wang, Hongning
通讯作者：
Wang, Hongning