Evaluation of cell types for assessment of cytogenetic damage in arsenic exposed population.
Evaluation of cell types for assessment of cytogenetic damage in arsenic exposed population.
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DOI：
10.1186/1476-4598-7-45
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发表时间：
2008-05-28
期刊：
影响因子：
37.3
通讯作者：
Giri AK
中科院分区：
文献类型：
--
作者：
Ghosh P;Basu A;Singh KK;Giri AK
细胞遗传学生物标志物对于评估环境暴露至关重要，并反映细胞损伤等对人类健康的不利影响。砷是一种潜在的断裂剂和非整倍体。一般来说，大多数关于砷致断裂作用的研究都是基于外周淋巴细胞、尿路上皮和口腔上皮细胞中微核（MN）频率的研究。为了找出最合适的细胞类型，我们在这里通过 MN 测定比较了 (a) 从文献综述中检索到的通过饮用水暴露于砷的不同人群的细胞遗传学损伤，以及 (b) 从我们自己的调查中发现的砷诱发的 Bowen 氏病患者。为了进行文献综述，我们使用以下关键词在 Pubmed 数据库中搜索了英语期刊文章：“砷”、“微核”、“饮用水”和“人类”的各种组合。我们选择了 13 项符合我们纳入标准的研究，这些研究测量了人类样本中上述三种细胞类型中的一种或多种的微核。与尿路上皮和颊粘膜细胞相比，通过暴露和未暴露的人之间的差异测量的中位效应大小，基于淋巴细胞的 MN 计数被发现更强。从 10 项研究中汇总得出的总体模式与我们自己的三项早期研究一致。与具有砷诱发的非癌性皮肤病变的对照个体相比，即使在砷诱发的鲍文病患者（病例）中，淋巴细胞的 MN 计数也更强。总体而言，可以得出结论，淋巴细胞中的 MN 在研究砷诱导的细胞遗传学损伤方面可能优于其他上皮细胞。
Cytogenetic biomarkers are essential for assessing environmental exposure, and reflect adverse human health effects such as cellular damage. Arsenic is a potential clastogen and aneugen. In general, the majority of the studies on clastogenic effects of arsenic are based on frequency of micronuclei (MN) study in peripheral lymphocytes, urothelial and oral epithelial cells. To find out the most suitable cell type, here, we compared cytogenetic damage through MN assay in (a) various populations exposed to arsenic through drinking water retrieved from literature review, as also (b) arsenic-induced Bowen's patients from our own survey. For literature review, we have searched the Pubmed database for English language journal articles using the following keywords: "arsenic", "micronuclei", "drinking water", and "human" in various combinations. We have selected 13 studies consistent with our inclusion criteria that measured micronuclei in either one or more of the above-mentioned three cell types, in human samples. Compared to urothelial and buccal mucosa cells, the median effect sizes measured by the difference between people with exposed and unexposed, lymphocyte based MN counts were found to be stronger. This general pattern pooled from 10 studies was consistent with our own set of three earlier studies. MN counts were also found to be stronger for lymphocytes even in arsenic-induced Bowen's patients (cases) compared to control individuals having arsenic-induced non-cancerous skin lesions. Overall, it can be concluded that MN in lymphocytes may be superior to other epithelial cells for studying arsenic-induced cytogenetic damage.
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影响因子：
6.4
作者：
Ghosh, P;Basu, A;Giri, AK
通讯作者：
Giri, AK
DOI：
10.1016/j.mrgentox.2007.05.005
发表时间：
2007-08-15
影响因子：
1.9
作者：
Ghosh, Pritha;Banerjee, Mayukh;Giri, Ashok K.
通讯作者：
Giri, Ashok K.
DOI：
10.1016/j.mrgentox.2004.07.008
发表时间：
2004-11-14
影响因子：
1.9
作者：
Martínez, V;Creus, A;Marcos, R
通讯作者：
Marcos, R
DOI：
10.1016/s1383-5742(03)00013-9
发表时间：
2003-03-01
影响因子：
5.3
作者：
Bonassi, S;Neri, M;Fenech, M
通讯作者：
Fenech, M
DOI：
10.1016/s1383-5742(97)00009-4
发表时间：
1997-06-01
影响因子：
5.3
作者：
Gonsebatt, ME;Vega, L;OstroskyWegman, P
通讯作者：
OstroskyWegman, P