Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones.
Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones.
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DOI：
10.1371/journal.pone.0282743
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
我们试图在微米量级上对草酸钙（CaOx）肾结石进行鉴定和定量分析，重点是对草酸钙一水（COM）和二水（COD）的定量鉴定。我们进行了傅里叶变换红外光谱（FTIR）、粉末x射线衍射（PXRD）和微聚焦x射线计算机断层扫描（microfocus x射线CT）测量，并比较了它们的结果。对聚焦于780 cm−1峰的FTIR光谱进行扩展分析，可以实现对COM/COD比的可靠分析。我们成功地用FTIR显微镜对肾结石薄片进行了定量分析，用微聚焦x射线CT系统对大样本进行了定量分析。基于微采样的PXRD测量、薄片的微观FTIR分析以及大体积肾结石样品的微聚焦x射线CT系统观察的分析结果大致一致，表明三种方法可以互补使用。这种定量分析方法评价了保存下来的石头表面的详细的草酸成分，提供了石头形成过程的信息。这一信息阐明了晶体在哪里和哪个相成核，晶体如何生长，以及如何从亚稳相过渡到稳定相。相变影响肾结石的生长速度和硬度，从而为肾结石的形成过程提供了重要线索。
We sought to identify and quantitatively analyze calcium oxalate (CaOx) kidney stones on the order of micrometers, with a focus on the quantitative identification of calcium oxalate monohydrate (COM) and dihydrate (COD). We performed Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (PXRD), and microfocus X-ray computed tomography measurements (microfocus X-ray CT) and compared their results. An extended analysis of the FTIR spectrum focusing on the 780 cm−1 peak made it possible to achieve a reliable analysis of the COM/COD ratio. We succeeded in the quantitative analysis of COM/COD in 50-μm2 areas by applying microscopic FTIR for thin sections of kidney stones, and by applying microfocus X-ray CT system for bulk samples. The analysis results based on the PXRD measurements with micro-sampling, the microscopic FTIR analysis of thin sections, and the microfocus X-ray CT system observation of a bulk kidney stone sample showed roughly consistent results, indicating that all three methods can be used complementarily. This quantitative analysis method evaluates the detailed CaOx composition on the preserved stone surface and provides information on the stone formation processes. This information clarifies where and which crystal phase nucleates, how the crystals grow, and how the transition from the metastable phase to the stable phase proceeds. The phase transition affects the growth rate and hardness of kidney stones and thus provides crucial clues to the kidney stone formation process.
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影响因子：
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作者：
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通讯作者：
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