BZR1 and BES1 participate in regulation of glucosinolate biosynthesis by brassinosteroids in Arabidopsis.
BZR1 and BES1 participate in regulation of glucosinolate biosynthesis by brassinosteroids in Arabidopsis.
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BZR1 和 BES1 参与拟南芥中油菜素类固醇生物合成的调节
DOI：
10.1093/jxb/ert094
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发表时间：
2013-05
影响因子：
6.9
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Guo R;Qian H;Shen W;Liu L;Zhang M;Cai C;Zhao Y;Qiao J;Wang Q
以油菜素内酯（BR）生物合成和信号转导以及硫苷生物合成相关的突变体和转基因植株为材料，研究了24-表油菜素内酯（EBR）对拟南芥硫苷生物合成的影响。结果表明，EBR显著降低了葡萄糖甙（S3）、萝卜甙（S4）、芥子甙（T4）等主要脂肪族硫代葡萄糖甙以及吲哚类硫代葡萄糖甙（IM）、新葡萄糖甙（1 IM）的含量。此外，BR缺失突变体cpd中硫代葡萄糖苷积累水平显著升高，而过表达BR生物合成基因DWF 4的转基因植株DWF 4-ox中硫代葡萄糖苷含量显著降低，证实了BR对硫代葡萄糖苷生物合成的抑制作用。BR信号转导受体BRI 1参与BR对硫苷生物合成的调控。35 S-BZR 1/bzr 1 -1D和bes 1-D植株中硫代葡萄糖苷含量和硫代葡萄糖苷生物合成基因的表达水平均低于相应的野生型植株，表明BR信号转导中的两个重要组分BZR 1和BES 1可能是BR抑制硫代葡萄糖苷生物合成的原因。BR对myb 28、myb 34和myb 122突变体中硫苷含量的抑制作用消失，表明这三个MYB因子对BR在硫苷生物合成中的调节是重要的。
The effect of 24-epibrassinolide (EBR) on glucosinolate biosynthesis in Arabidopsis thaliana was investigated in the present study by using mutants and transgenic plants involved in brassinosteroid (BR) biosynthesis and signal transduction, as well as glucosinolate biosynthesis. The results showed that EBR significantly decreased the contents of major aliphatic glucosinolates including glucoiberin (S3), glucoraphanin (S4), and glucoerucin (T4), as well as the indolic glucosinolates glucobrassicin (IM) and neoglucobrassicin (1IM). In addition, a significantly higher level of glucosinolates accumulated in the BR-deficient mutant cpd and a dramatically lower glucosinolate content in the transgenic plant DWF4-ox overexpressing the BR biosynthetic gene DWF4 compared with their related wild-types, confirmed the repressing effect of BR on glucosinolate biosynthesis. BRI1, the receptor of BR signal transduction, was involved in regulation of glucosinolate biosynthesis by BR. Furthermore, the observation of reduced content of glucosinolates and lower expression levels of glucosinolate biosynthetic genes in 35S-BZR1/bzr1-1D and bes1-D plants compared with the corresponding wild-types suggested that BZR1 and BES1, two important components in BR signal transduction, are responsible for the inhibiting role of BR in glucosinolate biosynthesis. The disappearance of the repressing effect of BR on glucosinolate content in the myb28, myb34, and myb122 mutants indicated that these three MYB factors are important for the regulation of BR in glucosinolate biosynthesis.
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影响因子：
9.4
作者：
Gigolashvili, Tamara;Engqvist, Martin;Fluegge, Ulf-Ingo
通讯作者：
Fluegge, Ulf-Ingo
影响因子：
7.4
作者：
Celenza, JL;Quiel, JA;Bender, J
通讯作者：
Bender, J
影响因子：
56.9
作者：
He, JX;Gendron, JM;Wang, ZY
通讯作者：
Wang, ZY
影响因子：
7.4
作者：
Goda, H;Shimada, Y;Yoshida, S
通讯作者：
Yoshida, S
影响因子：
7.2
作者：
Chen, SX;Glawischnig, E;Halkier, BA
通讯作者：
Halkier, BA