Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst Formation.
Effects of Melatonin on the Transcriptome of Human Granulosa Cells, Fertilization and Blastocyst Formation.
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褪黑素对人颗粒细胞转录组、受精和囊胚形成的影响。
DOI：
10.3390/ijms23126731
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发表时间：
2022-06-16
影响因子：
5.6
通讯作者：
Sugino, Norihiro
中科院分区：
文献类型：
--
作者：
Tamura, Isao;Tamura, Hiroshi;Kawamoto-Jozaki, Mai;Shirafuta, Yuichiro;Fujimura, Taishi;Doi-Tanaka, Yumiko;Mihara, Yumiko;Taketani, Toshiaki;Sugino, Norihiro
褪黑激素是一种很有前途的试剂，可以改善不孕症患者的辅助生殖技术（ART）结果。然而，褪黑激素并不是对所有不孕患者都有效，而且目前还不清楚褪黑激素对哪些患者有效。本研究探讨了褪黑激素对ART结果的影响，并探讨了其机制。褪黑激素增加了前一周期受精率低于50%的患者的受精率，但对前一周期受精率超过50%的患者没有影响。褪黑激素增加了前一周期胚胎发育率低于50%的患者的囊胚形成率，但对前一周期胚胎发育率超过50%的患者没有影响。为了阐明其机制，通过RNA序列检测患者颗粒细胞（GC）中褪黑激素治疗的转录组变化。褪黑激素治疗改变了ART结果较差的患者GC的转录组，使其与ART结果良好的患者的转录组相似。改变的基因与细胞死亡和T细胞活性的抑制，以及类固醇生成和血管生成的激活有关。褪黑激素治疗对前一个ART周期受精率和胚胎发育率差的患者有效。褪黑激素改变GC转录组，从而改变它们的功能，这可以改善卵母细胞的质量，从而获得良好的ART结果。
Melatonin is a promising reagent that can improve assisted reproductive technology (ART) outcomes in infertility patients. However, melatonin is not effective for all infertile patients, and it remains unclear for which patients melatonin would be effective. This study examined the effects of melatonin on ART outcomes and examined its mechanisms. Melatonin increased the fertilization rate in patients whose fertilization rates in the previous cycle were less than 50%, but not in patients whose fertilization rates were more than 50% in the previous cycle. Melatonin increased the blastocyst formation rate in patients whose embryo development rates in the previous cycle were less than 50%, but not in patients whose embryo development rates were more than 50% in the previous cycle. To clarify its mechanisms, transcriptome changes by melatonin treatment in granulosa cells (GCs) of the patients were examined by RNA-sequence. Melatonin treatment altered the transcriptomes of GCs of patients with poor ART outcomes so that they were similar to the transcriptomes of patients with good ART outcomes. The altered genes were associated with the inhibition of cell death and T-cell activity, and the activation of steroidogenesis and angiogenesis. Melatonin treatment was effective for patients with poor fertilization rates and poor embryo development rates in the previous ART cycle. Melatonin alters the GCs transcriptome and, thus, their functions, and this could improve the oocyte quality, leading to good ART outcomes.
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影响因子：
5.6
作者：
He C;Wang J;Zhang Z;Yang M;Li Y;Tian X;Ma T;Tao J;Zhu K;Song Y;Ji P;Liu G
通讯作者：
Liu G
影响因子：
12.8
作者：
Cheng JC;Fang L;Li Y;Wang S;Li Y;Yan Y;Jia Q;Wu Z;Wang Z;Han X;Sun YP
通讯作者：
Sun YP
影响因子：
3.6
作者：
Nenicu, Anca;Luers, Georg H.;Baumgart-Vogt, Eveline
通讯作者：
Baumgart-Vogt, Eveline
影响因子：
4.8
作者：
Chan, Olivia;Burke, J. Daniel;Fish, Eleanor N.
通讯作者：
Fish, Eleanor N.
影响因子：
2
作者：
Nishihara, Takuji;Hashimoto, Shu;Morimoto, Yoshiharu
通讯作者：
Morimoto, Yoshiharu