A Comparison of Traditional and Novel Methods for the Separation of Exosomes from Human Samples.
A Comparison of Traditional and Novel Methods for the Separation of Exosomes from Human Samples.
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DOI：
10.1155/2018/3634563
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发表时间：
2018
影响因子：
--
通讯作者：
Xiao MB
中科院分区：
文献类型：
--
作者：
Yu LL;Zhu J;Liu JX;Jiang F;Ni WK;Qu LS;Ni RZ;Lu CH;Xiao MB
外泌体是小（直径40-200 nm）膜囊泡的离散群体，其被大多数细胞类型释放到细胞外空间中，最终在循环中积累。作为分子信使，外泌体通过在不同细胞类型之间传递信息而发挥广泛的重要生理功能。由于这些功能特性，它们可能具有作为预后和诊断疾病的生物标志物来源的潜力。最近的研究发现，外泌体具有用作治疗靶点的药物递送剂的潜力。然而，对外来体的基础研究和对它们的治疗潜力的研究都需要存在有效和快速的方法来从人类样品中分离它们。在目前缺乏标准化方法的情况下，有几种方法可用于分离外泌体，但很少有研究以前比较了这些不同方法的效率和适用性。本文总结和比较了从人体样本中提取外泌体的传统和新型方法，并考虑了它们在临床实验室和床旁环境中使用的优缺点。
Exosomes are discrete populations of small (40-200 nm in diameter) membranous vesicles that are released into the extracellular space by most cell types, eventually accumulating in the circulation. As molecular messengers, exosomes exert a broad array of vital physiologic functions by transporting information between different cell types. Because of these functional properties, they may have potential as biomarker sources for prognostic and diagnostic disease. Recent research has found that exosomes have potential to be utilized as drug delivery agents for therapeutic targets. However, basic researches on exosomes and researches on their therapeutic potential both require the existence of effective and rapid methods for their separation from human samples. In the current absence of a standardized method, there are several methods available for the separation of exosomes, but very few studies have previously compared the efficiency and suitability of these different methods. This review summarized and compared the available traditional and novel methods for the extraction of exosomes from human samples and considered their advantages and disadvantages for use in clinical laboratories and point-of-care settings.
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影响因子：
5.4
作者：
Szatanek R;Baran J;Siedlar M;Baj-Krzyworzeka M
通讯作者：
Baj-Krzyworzeka M
影响因子：
21.3
作者：
Costa-Silva B;Aiello NM;Ocean AJ;Singh S;Zhang H;Thakur BK;Becker A;Hoshino A;Mark MT;Molina H;Xiang J;Zhang T;Theilen TM;García-Santos G;Williams C;Ararso Y;Huang Y;Rodrigues G;Shen TL;Labori KJ;Lothe IM;Kure EH;Hernandez J;Doussot A;Ebbesen SH;Grandgenett PM;Hollingsworth MA;Jain M;Mallya K;Batra SK;Jarnagin WR;Schwartz RE;Matei I;Peinado H;Stanger BZ;Bromberg J;Lyden D
通讯作者：
Lyden D
影响因子：
19.6
作者：
Alvarez, M. Lucrecia;Khosroheidari, Mahdieh;DiStefano, Johanna K.
通讯作者：
DiStefano, Johanna K.
一种基于 DNA 封端单壁碳纳米管的可见比色适体传感器，用于检测外泌体
影响因子：
12.6
作者：
Xia, Yaokun;Liu, Mengmeng;Chen, Jinghua
通讯作者：
Chen, Jinghua
影响因子：
17.1
作者：
Liu F;Vermesh O;Mani V;Ge TJ;Madsen SJ;Sabour A;Hsu EC;Gowrishankar G;Kanada M;Jokerst JV;Sierra RG;Chang E;Lau K;Sridhar K;Bermudez A;Pitteri SJ;Stoyanova T;Sinclair R;Nair VS;Gambhir SS;Demirci U
通讯作者：
Demirci U