Integration of flexible polyimide arrays into soft extracellular matrix-based hydrogel materials for a tissue-engineered electronic nerve interface (TEENI).
Integration of flexible polyimide arrays into soft extracellular matrix-based hydrogel materials for a tissue-engineered electronic nerve interface (TEENI).
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DOI：
10.1016/j.jneumeth.2020.108762
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发表时间：
2020-07-15
影响因子：
3
通讯作者：
Schmidt CE
中科院分区：
文献类型：
--
作者：
Spearman BS;Kuliasha CA;Judy JW;Schmidt CE
用于组织工程的仿生水凝胶可以改善组织再生并实现靶向细胞行为;人们越来越有兴趣将水凝胶与微电子技术相结合，以创建新的神经接口平台来帮助患者群体。然而，必须开发有效的工艺以成功地将柔性但相对刚性的（例如，1-10 GPa）微电子阵列内的软（例如，1-10 kPa）水凝胶。在这里，一种新的方法集成聚酰亚胺微电极阵列内的仿生水凝胶支架被证明用作组织工程电子神经接口（TEENI）。Tygon管和一系列3D打印模具用于促进水凝胶制造和可植入装置组装。其他可比较的再生周围神经接口技术不使用柔性微电极阵列设计，也不使用本文所述的水凝胶支架。这些方法通常使用刚性电极阵列，该刚性电极阵列固定到用作神经引导导管的类似刚性的可植入管。我们的研究结果表明，有一个实质性的机械之间的不匹配的柔性微电子阵列和软水凝胶。然而，使用这里描述的方法，存在适合于植入的这些再生周围神经界面的一致制造。所开发的组装过程导致微电极阵列在软组织工程化水凝胶内的可重复和一致的集成。如其他地方所报道的，这些装置已成功植入大鼠坐骨神经模型并产生神经记录。这个过程可以适用于其他应用和水凝胶，其中柔性电子材料与软再生支架相结合。
Biomimetic hydrogels used in tissue engineering can improve tissue regeneration and enable targeted cellular behavior; there is growing interest in combining hydrogels with microelectronics to create new neural interface platforms to help patient populations. However, effective processes must be developed to successfully integrate flexible but relatively stiff (e.g., 1–10 GPa) microelectronic arrays within soft (e.g., 1–10 kPa) hydrogels. Here, a novel method for integrating polyimide microelectrode arrays within a biomimetic hydrogel scaffold is demonstrated for use as a tissue-engineered electronic nerve interface (TEENI). Tygon tubing and a series of 3D printed molds were used to facilitate hydrogel fabrication and implantable device assembly. Other comparable regenerative peripheral nerve interface technologies do not utilize the flexible microelectrode array design nor the hydrogel scaffold described here. These methods typically use stiff electrode arrays that are affixed to a similarly stiff implantable tube serving as the nerve guidance conduit. Our results indicate that there is a substantial mechanical mismatch between the flexible microelectronic arrays and the soft hydrogel. However, using the methods described here, there is consistent fabrication of these regenerative peripheral nerve interfaces suitable for implantation. The assembly process that was developed resulted in repeatable and consistent integration of microelectode arrays within a soft tissue-engineered hydrogel. As reported elsewhere, these devices have been successfully implanted in a rat sciatic nerve model and yielded neural recordings. This process can be adapted for other applications and hydrogels in which flexible electronic materials are combined with soft regenerative scaffolds.
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DOI：
10.3389/neuro.16.005.2009
发表时间：
2009
期刊：
Frontiers in neuroengineering
影响因子：
--
作者：
Garde K;Keefer E;Botterman B;Galvan P;Romero MI
通讯作者：
Romero MI
影响因子：
5.3
作者：
Labrador, RO;Butí, M;Navarro, X
通讯作者：
Navarro, X
DOI：
10.1002/jbm.a.36814
发表时间：
2020-03
期刊：
Journal of biomedical materials research. Part A
影响因子：
--
作者：
Spearman BS;Agrawal NK;Rubiano A;Simmons CS;Mobini S;Schmidt CE
通讯作者：
Schmidt CE
影响因子：
19
作者：
Spearman, Benjamin S;Desai, Vidhi H;Schmidt, Christine E
通讯作者：
Schmidt, Christine E
影响因子：
3
作者：
Pierce, Andrew L.;Sommakia, Salah;Otto, Kevin J.
通讯作者：
Otto, Kevin J.