Topical injury evaluation of the murine colorectal mucosa using confocal endomicrosopy: a valuable method for assessing mucosal injuries associated with risk of pathogen transmission.
Topical injury evaluation of the murine colorectal mucosa using confocal endomicrosopy: a valuable method for assessing mucosal injuries associated with risk of pathogen transmission.
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使用共聚焦内分子膜对鼠结直肠粘膜的局部损伤评估：一种评估与病原体传播风险相关的粘膜损伤的宝贵方法。
DOI：
10.1111/jmi.12438
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发表时间：
2016-11
影响因子：
2
通讯作者：
Motamedi M
中科院分区：
文献类型：
--
作者：
Vargas G;Vincent KL;Wei J;Bourne N;Motamedi M
关于上皮损伤对结直肠表面的影响以及对性传播感染传播的可能影响的担忧提示需要在检查此类损伤的临床前研究中评价粘膜显微镜表面的方法。这包括确定局部HIV预防产品对粘膜屏障完整性的影响。使用高分辨率显微内镜的体内成像可以显示由损伤/表面创伤引起的粘膜屏障缺陷。共聚焦显微内镜进行了研究，以评估的能力，图像表面损伤所造成的局部应用的化学品中使用的润滑剂和微生物产品。用50 μl直肠剂量的0.2%苯扎氯铵（BZK）溶液、1% BZK或磷酸盐缓冲盐水对照处理小鼠20分钟，使用共聚焦显微内镜（CE）进行体内成像，以评估上皮破坏。成像后，处死小鼠并通过组织学评价直肠组织。共聚焦图像根据隐窝和上皮微结构的破坏程度进行分级。根据染色切片中观察到的上皮破坏百分比对组织学进行分级。共焦图像的特点，证实了高分辨率双光子显微镜。基于体内CE图像的定量分级，在用BZK处理后观察到微观尺度的破坏，随着剂量的增加而发生损伤。在CE中观察到管腔表面的上皮破裂，隐窝之间明显，管腔侧的隐窝结构改变，并通过组织学证实。CE的高分辨率成像可用作一种非侵入性工具，用于在表面损伤后快速目视评估直肠上皮完整性，可能提供有价值的上皮损伤或创伤指征。
Concern regarding the effect of epithelial damage to the colorectal surface and possible impact on sexually transmitted infection transmission prompts the need for methods to evaluate the mucosal microscopic surface in preclinical studies examining such injury. This includes determining the effect of topical HIV prevention products on mucosal barrier integrity. In vivo imaging with high resolution endomicroscopy could reveal defects in the mucosal barrier resulting from injury/surface trauma. Confocal endomicroscopy was investigated to assess the ability to image surface injury resulting from topical application of a chemical used in lubricants and microbial products. Mice treated with a 50 μl rectal dose of 0.2% benzalkonium chloride (BZK) solution, 1% BZK, or phosphate-buffered saline control for 20 minutes were imaged in vivo using confocal endomicroscopy (CE) for assessment of epithelial disruption. Following imaging, mice were sacrificed and rectal tissue evaluated by histology. Confocal images were graded based on degree of disruption to crypt and epithelial microstructure. Histology was graded based on percent of epithelial disruption observed in stained sections. Confocal image features were confirmed by high resolution two-photon microscopy. Based on quantitative grading of in vivo CE images, disruption at the microscopic scale was observed following treatment with BZK, with increased injury occurring with higher dose. Epithelial disruption at the lumen surface, evident between crypts and alteration in crypt structure on the luminal side were observed in CE and confirmed by histology. High resolution imaging by CE can be used as a noninvasive tool for rapid visual assessment of rectal epithelial integrity following surface injury, potentially providing valuable indication of epithelial injury or trauma.
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DOI：
10.1097/qai.0b013e3182898392
发表时间：
2013-07-01
期刊：
Journal of acquired immune deficiency syndromes (1999)
影响因子：
--
作者：
McElrath MJ;Smythe K;Randolph-Habecker J;Melton KR;Goodpaster TA;Hughes SM;Mack M;Sato A;Diaz G;Steinbach G;Novak RM;Curlin ME;Lord JD;Maenza J;Duerr A;Frahm N;Hladik F;NIAID HIV Vaccine Trials Network
通讯作者：
NIAID HIV Vaccine Trials Network
影响因子：
4.3
作者：
De Palma, Giovanni D.
通讯作者：
De Palma, Giovanni D.
DOI：
10.7196/samj.7002
发表时间：
2014-01-01
期刊：
SAMJ: South African Medical Journal
影响因子：
--
作者：
Rebe, K B;de Swardt, G;McIntyre, J A
通讯作者：
McIntyre, J A
影响因子：
2.9
作者：
Phillips, DM;Taylor, CL;Maguire, RA
通讯作者：
Maguire, RA
影响因子：
3.1
作者：
Gorbach PM;Weiss RE;Fuchs E;Jeffries RA;Hezerah M;Brown S;Voskanian A;Robbie E;Anton P;Cranston RD
通讯作者：
Cranston RD