Differential effects of ethanol consumption on synthesis of cytoplasmic and mitochondrial encoded subunits of the ATP synthase.
Differential effects of ethanol consumption on synthesis of cytoplasmic and mitochondrial encoded subunits of the ATP synthase.
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乙醇消耗对细胞质和线粒体编码的 ATP 合酶亚基合成的不同影响。
DOI：
10.1111/j.1530-0277.1994.tb00064.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Cunningham,CC
中科院分区：
文献类型：
--
作者：
Coleman,WB;Cahill,A;Ivester,P;Cunningham,CC
在从乙醇喂养大鼠和对照大鼠肝脏制备的线粒体和亚线粒体颗粒中测定线粒体F0.F1-ATP合酶几个亚基的相对浓度。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离多肽，并通过光密度法分析染色的凝胶中ATP合酶亚基的相对浓度。在乙醇喂养动物的线粒体和亚线粒体颗粒中观察到线粒体基因产物ATP酶亚基8的相对浓度显著降低。另一个线粒体编码的ATP酶亚基ATP酶6的相对浓度也被抑制，这在亚线粒体颗粒中得到证实。相比之下，在F0的亚基α、β和OSCP中没有显著的乙醇相关抑制。F1或完整线粒体中的腺嘌呤核苷酸载体。这些结果表明，乙醇消耗导致线粒体合成的亚基6和8的含量减少，而没有影响施加在ATP合酶复合物的核基因产物的浓度。同样，腺嘌呤核苷酸转运蛋白，也是一种核基因产物，不受乙醇消耗的影响。
The relative concentrations of several subunits of the mitochondrial F0.F1‐ATP synthase were determined in mitochondria and submitochondrial particles prepared from the livers of ethanol‐fed and control rats. The polypeptides were separated by sodium dodecylsulfate‐polyacrylamide gel electrophoresis and the stained gels were analyzed by densitometry for the relative concentrations of the ATP synthase subunits. A significant decrease in the relative concentration of the mitochondrial gene product, ATPase subunit 8, was observed in mitochondria and submitochondrial particles from ethanol‐fed animals. The relative concentration of the other mitochondrial encoded ATPase subunit, ATPase 6, was also depressed, as confirmed in submitochondrial particles. In contrast, there were no significant ethanol‐related depressions in subunits α, β, and OSCP of the F0. F1or the adenine nucleotide carrier in intact mitochondria. These results demonstrate that ethanol consumption causes a decrease in the content of mitochondrial synthesized subunits 6 and 8 whereas no effect is exerted on the concentrations of nuclear gene products of the ATP synthase complex. Likewise, the adenine nucleotide transporter, also a nuclear gene product, is unaffected by ethanol consumption.
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影响因子：
11.4
作者：
FEARNLEY, IM;WALKER, JE
通讯作者：
WALKER, JE
乙醇诱导脂肪肝大鼠肝线粒体腺嘌呤核苷酸代谢的控制。
影响因子：
4.1
作者：
P. I. Spach;R. Bottenus;C. Cunningham
通讯作者：
C. Cunningham
长期服用乙醇会导致肝脏代谢发生变化。
影响因子：
4.1
作者：
J. Bernstein;L. Videla;Y. Israel
通讯作者：
Y. Israel
长期服用乙醇会导致肝脏代谢改变。
影响因子：
4.1
作者：
L. Videla;J. Bernstein;Y. Israel
通讯作者：
Y. Israel
线粒体 DNA URFA6L 产物 Chargerin II 在大鼠肝线粒体能量转导中作用的免疫化学研究。
DOI：
10.1016/0006-291x(87)90739-x
发表时间：
1987
影响因子：
3.1
作者：
J. Uchida;M. Takigawa;T. Yamane;T. Negama;I. Tani;T. Higuti
通讯作者：
T. Higuti