Matrix proteins are inefficiently imported into Arabidopsis peroxisomes lacking the receptor-docking peroxin PEX14.
Matrix proteins are inefficiently imported into Arabidopsis peroxisomes lacking the receptor-docking peroxin PEX14.
复制标题
DOI：
10.1007/s11103-011-9782-0
复制
发表时间：
2011-09
影响因子：
5.1
通讯作者：
Bartel, Bonnie
中科院分区：
文献类型：
--
作者：
Monroe-Augustus, Melanie;Ramon, Naxhiely Martinez;Ratzel, Sarah E.;Lingard, Matthew J.;Christensen, Sarah E.;Murali, Chaya;Bartel, Bonnie
过氧化物酶体生物合成蛋白（过氧化物酶）的突变可导致各种真核生物的发育缺陷。PEX 14和PEX 13是参与在过氧化物酶体膜上对接货物-受体复合物的过氧化物，从而有助于货物转运到过氧化物酶体基质中。遗传筛选已经揭示了许多拟南芥过氧化物酶在过氧化物酶体基质蛋白的输入作用;通过这些筛选分离的可行等位基因通常是部分功能丧失的等位基因，而破坏基质蛋白向过氧化物酶体的递送的无效突变可以赋予胚胎致死性。在这项研究中，我们使用正向和反向遗传学在拟南芥分离pex 14等位基因。我们发现，所有四个等位基因赋予减少PEX 14 mRNA水平，并显示生理和分子缺陷，这表明减少，但没有取消过氧化物酶体基质蛋白的输入。最不严重的pex 14等位基因，pex 14 -3，积累低水平的C-末端截短的PEX 14产品，保留部分功能。令人惊讶的是，即使是严重的pex 14 -2等位基因，缺乏可检测的PEX 14 mRNA和PEX 14蛋白，是可行的，可育的，并显示残留的过氧化物酶体基质蛋白输入。随着pex 14植物的成熟，进口有所改善。总之，我们的数据表明，PEX 14促进，但不是必不可少的过氧化物酶体基质蛋白在植物中的进口。
Mutations in peroxisome biogenesis proteins (peroxins) can lead to developmental deficiencies in various eukaryotes. PEX14 and PEX13 are peroxins involved in docking cargo-receptor complexes at the peroxisomal membrane, thus aiding in the transport of the cargo into the peroxisomal matrix. Genetic screens have revealed numerous Arabidopsis thaliana peroxins acting in peroxisomal matrix protein import; the viable alleles isolated through these screens are generally partial loss-of-function alleles, whereas null mutations that disrupt delivery of matrix proteins to peroxisomes can confer embryonic lethality. In this study, we used forward and reverse genetics in Arabidopsis to isolate four pex14 alleles. We found that all four alleles conferred reduced PEX14 mRNA levels and displayed physiological and molecular defects suggesting reduced but not abolished peroxisomal matrix protein import. The least severe pex14 allele, pex14-3, accumulated low levels of a C-terminally truncated PEX14 product that retained partial function. Surprisingly, even the severe pex14-2 allele, which lacked detectable PEX14 mRNA and PEX14 protein, was viable, fertile, and displayed residual peroxisome matrix protein import. As pex14 plants matured, import improved. Together, our data indicate that PEX14 facilitates, but is not essential for peroxisomal matrix protein import in plants.
登录
查看更多内容
影响因子：
4.8
作者：
Hayashi, M;Yagi, M;Nishimura, M
通讯作者：
Nishimura, M
影响因子：
56.9
作者：
Hu, JP;Aguirre, M;Chory, J
通讯作者：
Chory, J
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
4.4
作者：
BELL, CJ;ECKER, JR
通讯作者：
ECKER, JR
影响因子：
64.5
作者：
Albertini, M;Rehling, P;Kunau, WH
通讯作者：
Kunau, WH