Pulsed electromagnetic fields improve bone microstructure and strength in ovariectomized rats through a Wnt/Lrp5/β-catenin signaling-associated mechanism.
Pulsed electromagnetic fields improve bone microstructure and strength in ovariectomized rats through a Wnt/Lrp5/β-catenin signaling-associated mechanism.
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DOI：
10.1371/journal.pone.0079377
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Luo E
中科院分区：
文献类型：
--
作者：
Jing D;Li F;Jiang M;Cai J;Wu Y;Xie K;Wu X;Tang C;Liu J;Guo W;Shen G;Luo E
脉冲电磁场（PEMF）作为一种非侵入性的成骨方法，在体内外均能促进成骨。然而，PEMF治疗骨量减少/骨质疏松症的确切机制尚不清楚，这进一步限制了PEMF的广泛临床应用。本研究系统研究了PEMF对去卵巢（OVX）大鼠骨质疏松骨微结构和骨质量的影响及其相关信号通路机制。将30只大鼠平均分配到对照组、OVX组和OVX + PEMF组。OVX + PEMF组每天接受15 Hz，2.4 mT（峰值）的PEMF暴露8 h。10周后，OVX + PEMF组表现出显著改善的骨量和骨结构，通过MicroCT分析中BMD、Tb. N、Tb. Th和BV/TV的增加以及Tb. Sp和SMI水平的抑制来证明。三点弯曲试验表明，PEMF衰减OVX大鼠股骨的生物力学强度恶化，证明增加最大载荷和弹性模量。RT-PCR分析表明，PEMF暴露显著促进了经典Wnt信号通路中Wnt1、LRP 5和β-catenin的整体基因表达，但对RANKL或RANK基因表达无明显影响。总之，我们目前的研究结果强调，PEMF通过促进Wnt/LRP5/β-catenin信号的激活而不是通过抑制RANKL-RANK信号来减轻OVX诱导的大鼠骨微结构和强度恶化。本研究丰富了我们对PEMF成骨活性的基础认识，并可能使PEMF在抑制骨量减少/骨质疏松症方面的临床应用更加有效和科学。
Growing evidence has demonstrated that pulsed electromagnetic field (PEMF), as an alternative noninvasive method, could promote remarkable in vivo and in vitro osteogenesis. However, the exact mechanism of PEMF on osteopenia/osteoporosis is still poorly understood, which further limits the extensive clinical application of PEMF. In the present study, the efficiency of PEMF on osteoporotic bone microarchitecture and bone quality together with its associated signaling pathway mechanisms was systematically investigated in ovariectomized (OVX) rats. Thirty rats were equally assigned to the Control, OVX and OVX+PEMF groups. The OVX+PEMF group was subjected to daily 8-hour PEMF exposure with 15 Hz, 2.4 mT (peak value). After 10 weeks, the OVX+PEMF group exhibited significantly improved bone mass and bone architecture, evidenced by increased BMD, Tb.N, Tb.Th and BV/TV, and suppressed Tb.Sp and SMI levels in the MicroCT analysis. Three-point bending test suggests that PEMF attenuated the biomechanical strength deterioration of the OVX rat femora, evidenced by increased maximum load and elastic modulus. RT-PCR analysis demonstrated that PEMF exposure significantly promoted the overall gene expressions of Wnt1, LRP5 and β-catenin in the canonical Wnt signaling, but did not exhibit obvious impact on either RANKL or RANK gene expressions. Together, our present findings highlight that PEMF attenuated OVX-induced deterioration of bone microarchitecture and strength in rats by promoting the activation of Wnt/LRP5/β-catenin signaling rather than by inhibiting RANKL-RANK signaling. This study enriches our basic knowledge to the osteogenetic activity of PEMF, and may lead to more efficient and scientific clinical application of PEMF in inhibiting osteopenia/osteoporosis.
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影响因子：
5.6
作者：
Kim, Tae-Ho;Jung, Ji Won;Kim, Shin-Yoon
通讯作者：
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影响因子：
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作者：
Assiotis A;Sachinis NP;Chalidis BE
通讯作者：
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影响因子：
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作者：
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脉冲电磁场对链脲佐菌素治疗大鼠糖尿病骨丢失的预防作用
影响因子：
4
作者：
Jing, D.;Cai, J.;Xu, Q.
通讯作者：
Xu, Q.
影响因子：
4
作者：
Laib, A;Kumer, JL;Lane, NE
通讯作者：
Lane, NE