Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine-Quantum Dots.
Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine-Quantum Dots.
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DOI：
10.1021/jz100248c
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Payne CK
中科院分区：
文献类型：
--
作者：
Jablonski AE;Kawakami T;Ting AY;Payne CK
量子点的细胞内、细胞质递送是细胞成像的重要目标。最近，通过共焦显微镜表征，疏水性阴离子芘丁酸被提议用作阳离子量子点的递送剂。使用细胞外量子点猝灭剂 QSY-21 作为共焦显微镜的替代品，我们证明量子点保留在细胞表面，并且在芘丁酸处理后不会穿过质膜，这一结果已通过透射电子显微镜得到证实。芘丁酸导致量子点的细胞结合增加，而不是细胞内递送。这些结果表征了 QSY-21 作为量子点猝灭剂的用途，并强调了使用共焦显微镜时使用互补技术的重要性。
The intracellular, cytosolic, delivery of quantum dots is an important goal for cellular imaging. Recently, a hydrophobic anion, pyrenebutyrate had been proposed to serve as a delivery agent for cationic quantum dots as characterized by confocal microscopy. Using an extracellular quantum dot quencher, QSY-21, as an alternative to confocal microscopy, we demonstrate that quantum dots remain on the cell surface and do not cross the plasma membrane following pyrenebutyrate treatment, a result that is confirmed with transmission electron microscopy. Pyrenebutyrate leads to increased cellular binding of quantum dots rather than intracellular delivery. These results characterize the use of QSY-21 as a quantum dot quencher and highlight the importance of the use of complementary techniques when using confocal microscopy.
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