Cytotoxicity of free versus multilayered polyelectrolytes.
Cytotoxicity of free versus multilayered polyelectrolytes.
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DOI：
10.1021/bm201142x
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发表时间：
2011-11-14
影响因子：
6.2
通讯作者：
Schlenoff JB
中科院分区：
文献类型：
--
作者：
Martinez JS;Keller TC 3rd;Schlenoff JB
用大鼠平滑肌A7r5细胞和人骨肉瘤U-2OS细胞评价了聚电解质多层膜(PEMU)的细胞毒性。通过细胞生长、活力和代谢试验比较两种细胞对聚丙烯酸和聚烯丙基胺盐酸盐的反应，在浓度达到10 mM和不同厚度的(PAA/PAH)PEMU中。对于两种细胞类型，细胞毒性与溶液聚电解质浓度的增加有关，且正电荷的PAH的细胞毒性大于负电荷的PAA。虽然两种细胞在PEMU上的代谢和增殖都比在组织培养塑料上慢，但几乎没有观察到对细胞直接毒性的证据。事实上，在PAH终止的PEMU中观察到了更广泛的黏附和细胞骨架组织的证据。不同厚度的PEMU表面细胞活性和存活率的差异主要是由于这些黏附依赖细胞系的附着不同所致。
The cytotoxicity of polyelectrolytes commonly employed for layer-by-layer deposition of polyelectrolyte multilayers (PEMUs) was assessed using rat smooth muscle A7r5 and human osteosarcoma U-2 OS cells. Cell growth, viability, and metabolic assays were used to compare the responses of both cell lines to poly(acrylic acid), PAA, and poly(allylamine hydrochloride), PAH, in solution at concentrations up to 10 mM and to varying thicknesses of (PAA/PAH) PEMUs. Cytotoxicity correlated with increasing concentration of solution polyelectrolytes for both cell types and was greater for the positively-charged PAH than for the negatively-charged PAA. While metabolism and proliferation of both cell types was slower on PEMUs than on tissue culture plastic, little evidence for direct toxicity on cells was observed. In fact, evidence for more extensive adhesion and cytoskeletal organization was observed with PAH-terminated PEMUs. Differences in cell activity and viability on different thickness PEMU surfaces resulted primarily from differences in attachment for these adhesion-dependent cell lines.
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影响因子：
4.9
作者：
Chua, P. H.;Neoh, K. G.;Kang, E. T.
通讯作者：
Kang, E. T.
影响因子：
3.9
作者：
Kadowaki, Koji;Matsusaki, Michiya;Akashi, Mitsuru
通讯作者：
Akashi, Mitsuru
影响因子：
--
作者：
Ingber, Donald E.;Mow, Van C.;Vunjak-Novakovic, Gordana
通讯作者：
Vunjak-Novakovic, Gordana
影响因子：
5.5
作者：
Dubas, ST;Schlenoff, JB
通讯作者：
Schlenoff, JB
影响因子：
3.2
作者：
Hamid, R;Rotshteyn, Y;Bullock, P
通讯作者：
Bullock, P