Mineralized gelatin methacrylate-based matrices induce osteogenic differentiation of human induced pluripotent stem cells.
Mineralized gelatin methacrylate-based matrices induce osteogenic differentiation of human induced pluripotent stem cells.
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DOI：
10.1016/j.actbio.2014.08.010
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发表时间：
2014-12
影响因子：
9.7
通讯作者：
Varghese, Shyni
中科院分区：
文献类型：
--
作者：
Kang, Heemin;Shih, Yu-Ru V.;Hwang, Yongsung;Wen, Cai;Rao, Vikram;Seo, Timothy;Varghese, Shyni
关键词：
人类诱导多能干细胞（hiPSC）是一种具有多能性和自我更新特性的有前途的细胞来源。设计简单而坚固的生物材料，具有诱导 hiPSC 谱系特异性的先天能力，有助于实现其在再生医学中的应用。在这项研究中，我们研究了含有磷酸钙矿物质的生物材料诱导 hiPSC 成骨分化的潜力。使用矿化明胶甲基丙烯酸酯基质培养的 hiPSC 在二维 (2-D) 和三维 (3-D) 培养物中，在不含任何成骨诱导化学成分或生长因子的生长培养基中进行离体成骨分化。我们的研究结果表明，仅通过基于生物材料的线索即可实现 hiPSC 的成骨分化，这为个性化再生医学提供了新途径。这种生物材料不仅可以充当结构支架，还可以提供组织特异性功能，例如指导干细胞分化定向，在骨组织工程中具有巨大潜力。
Human induced pluripotent stem cells (hiPSCs) are a promising cell source with pluripotency and self-renewal properties. Design of simple and robust biomaterials with an innate ability to induce lineage-specificity of hiPSCs is desirable to realize their applications in regenerative medicine. In this study, we investigated the potential of biomaterials containing calcium phosphate minerals to induce osteogenic differentiation of hiPSCs. hiPSCs cultured using mineralized gelatin methacrylate-based matrices underwent osteogenic differentiation ex vivo, both in two- dimensional (2-D) and three-dimensional (3-D) cultures, in growth medium devoid of any osteogenic-inducing chemical components or growth factors. Our findings that osteogenic differentiation of hiPSCs can be achieved through biomaterial-based cues alone present new avenues for personalized regenerative medicine. Such biomaterials that could not only act as structural scaffolds, but could also provide tissue-specific functions such as directing stem cell differentiation commitment, have great potential in bone tissue engineering.
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影响因子：
14
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
工程细胞材料界面，用于人类多能干细胞长期扩张。
影响因子：
14
作者：
Chang CW;Hwang Y;Brafman D;Hagan T;Phung C;Varghese S
通讯作者：
Varghese S
影响因子：
14
作者：
Bryant, Stephanie J.;Cuy, Janet L.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
影响因子：
2.9
作者：
HEINONEN, JK;LAHTI, RJ
通讯作者：
LAHTI, RJ
影响因子：
--
作者：
Hwang, Yongsung;Zhang, Chao;Varghese, Shyni
通讯作者：
Varghese, Shyni