Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis.
Effects of Luteolin on Biofilm of Trueperella pyogenes and Its Therapeutic Effect on Rat Endometritis.
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DOI：
10.3390/ijms232214451
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发表时间：
2022-11-21
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
化脓性真杆菌是一种机会致病菌，可引起动物化脓性感染。新型抗生物被膜药物的开发将改善目前控制T。畜牧业中的化脓性感染。木犀草素是一种具有抗菌特性的天然黄酮类化合物。本研究探讨了木犀草素对T。化脓菌生物膜进行分析和探讨。木樨草素对T.化脓性链球菌分别为156 μg/mL和312 μg/mL。用激光共聚焦显微镜和扫描电镜观察毛地黄黄酮的抗生物膜作用。结果表明，312 μg/mL的木犀草素作用8 h后，可使大块生物膜分散成小团。实时荧光定量PCR检测结果显示，木犀草素能显著抑制生物被膜相关基因luxS、plo、rbsB和lsrB的相对表达。此外，毛地黄黄酮对T.用冰醋酸刺激法建立大鼠化脓性肠炎模型，化脓性子宫内输注。我们的研究表明，木犀草素可以显着减轻大鼠肠炎的症状。这些结果可为毛地黄黄酮等黄酮类化合物的临床治疗提供新的思路。化脓菌生物膜相关感染。
Trueperella pyogenes is an opportunistic pathogen that causes suppurative infections in animals. The development of new anti-biofilm drugs will improve the current treatment status for controlling T. pyogenes infections in the animal husbandry industry. Luteolin is a naturally derived flavonoid compound with antibacterial properties. In this study, the effects and the mechanism of luteolin on T. pyogenes biofilm were analyzed and explored. The MBIC and MBEC of luteolin on T. pyogenes were 156 μg/mL and 312 μg/mL, respectively. The anti-biofilm effects of luteolin were also observed by a confocal laser microscope and scanning electron microscope. The results indicated that 312 μg/mL of luteolin could disperse large pieces of biofilm into small clusters after 8 h of treatment. According to the real-time quantitative PCR detection results, luteolin could significantly inhibit the relative expression of the biofilm-associated genes luxS, plo, rbsB and lsrB. In addition, the in vivo anti-biofilm activity of luteolin against T. pyogenes was studied using a rat endometritis model established by glacial acetic acid stimulation and T. pyogenes intrauterine infusion. Our study showed that luteolin could significantly reduce the symptoms of rat endometritis. These data may provide new opinions on the clinical treatment of luteolin and other flavonoid compounds on T. pyogenes biofilm-associated infections.
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影响因子：
5.2
作者：
Fu Y;Wang W;Zeng Q;Wang T;Qian W
通讯作者：
Qian W
影响因子：
3.1
作者：
Shao, Hanjuan;Lamont, Richard J.;Demuth, Donald R.
通讯作者：
Demuth, Donald R.
影响因子：
2.8
作者：
Ernstberger, M.;Oehl, H.;Bollwein, H.
通讯作者：
Bollwein, H.
DOI：
10.1292/jvms.16-0401
发表时间：
2017-03-23
期刊：
The Journal of veterinary medical science
影响因子：
--
作者：
Ishiyama D;Mizomoto T;Ueda C;Takagi N;Shimizu N;Matsuura Y;Makuuchi Y;Watanabe A;Shinozuka Y;Kawai K
通讯作者：
Kawai K
影响因子：
5.4
作者：
Carbone A;Parrino B;Cusimano MG;Spanò V;Montalbano A;Barraja P;Schillaci D;Cirrincione G;Diana P;Cascioferro S
通讯作者：
Cascioferro S