Biases in ion transmission through an electrospray ionization-mass spectrometry capillary inlet.
Biases in ion transmission through an electrospray ionization-mass spectrometry capillary inlet.
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DOI：
10.1016/j.jasms.2009.08.018
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发表时间：
2009-12
影响因子：
3.2
通讯作者：
Smith RD
中科院分区：
文献类型：
--
作者：
Page JS;Marginean I;Baker ES;Kelly RT;Tang K;Smith RD
一个加热毛细管入口的电喷雾电离质谱（ESI-MS）接口进行了比较，较短版本的入口，以确定低流量（纳米）电喷雾传输和电离效率的影响。五种不同的入口长度进行了研究，从6.4到1.3厘米。正如预期的那样，电喷雾电流传输效率随着毛细管长度的减小而增加，这是由于毛细管内壁的损失减少。较短入口的传输效率的这种增加与电喷雾液滴的去溶剂化减少相结合。令人惊讶的是，随着入口长度的减少，一些分析物显示出很少或没有增加的灵敏度，而另一些显示出高达15倍的增益。的变化被证明击败至少部分相关的分析物的迁移率，观察到的最大收益较高的流动性物种，但也受到溶液的电导率，流速和入口温度。提出了最大限度地提高灵敏度，同时最大限度地减少通过加热毛细管界面的离子传输的偏差的策略。
A heated capillary inlet for an electrospray ionization mass spectrometry (ESI-MS) interface was compared with shorter versions of the inlet to determine the effects on transmission and ionization efficiencies for low-flow (nano) electrosprays. Five different inlet lengths were studied, ranging from 6.4 to 1.3 cm. As expected, the electrospray current transmission efficiency increased with decreasing capillary length due to reduced losses to the inside walls of the capillary. This increase in transmission efficiency with shorter inlets was coupled with reduced desolvation of electrosprayed droplets. Surprisingly, as the inlet length was decreased, some analytes showed little or no increase in sensitivity, while others showed as much as a15 – fold gain. The variation was shown to beat least partially correlated with analyte mobilities, with the largest gains observed for higher mobility species, but also affected by solution conductivity, flow rate, and inlet temperature. Strategies for maximizing sensitivity while minimizing biases in ion transmission through the heated capillary interface are proposed.
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影响因子：
7.4
作者：
Wilm, M;Mann, M
通讯作者：
Mann, M
影响因子：
7.4
作者：
Shen, Y;Tolic, N;Smith, RD
通讯作者：
Smith, RD
DOI：
10.1016/j.jasms.2007.05.018
发表时间：
2007-09-01
影响因子：
3.2
作者：
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通讯作者：
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影响因子：
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作者：
WILM, MS;MANN, M
通讯作者：
MANN, M
影响因子：
2
作者：
GALE, DC;SMITH, RD
通讯作者：
SMITH, RD