Protective Effect of Edaravone on Glutamate-Induced Neurotoxicity in Spiral Ganglion Neurons.
Protective Effect of Edaravone on Glutamate-Induced Neurotoxicity in Spiral Ganglion Neurons.
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依达拉奉对谷氨酸诱导的螺旋神经节神经毒性的保护作用
DOI：
10.1155/2016/4034218
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发表时间：
2016
影响因子：
3.1
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Bai X;Zhang C;Chen A;Liu W;Li J;Sun Q;Wang H
谷氨酸是哺乳动物大脑中一种重要的兴奋性神经递质，但过量的谷氨酸可引起兴奋性毒性，导致神经元死亡。螺旋神经节神经元作为双极神经元，起着将听觉信息从耳朵传递到大脑的桥梁作用，谷氨酸过量可导致感音神经性听力损失。在本研究中，自由基清除剂依达拉奉对谷氨酸诱导的SGN细胞损伤具有预防和治疗作用，通过四甲基偶氮唑盐比色法和台盼蓝染色检测。Ho.33342和PI双重染色显示谷氨酸处理过程中出现细胞凋亡和坏死，细胞死亡以细胞凋亡为主。氧化应激在谷氨酸诱导的细胞损伤中起重要作用，但依达拉奉预处理可减轻细胞死亡。Western印迹结果表明，谷氨酸的毒性和依达拉奉保护作用的机制与PI3K通路和Bcl2蛋白家族有关。
Glutamate is an important excitatory neurotransmitter in mammalian brains, but excessive amount of glutamate can cause “excitotoxicity” and lead to neuronal death. As bipolar neurons, spiral ganglion neurons (SGNs) function as a “bridge” in transmitting auditory information from the ear to the brain and can be damaged by excessive glutamate which results in sensorineural hearing loss. In this study, edaravone, a free radical scavenger, elicited both preventative and therapeutic effects on SGNs against glutamate-induced cell damage that was tested by MTT assay and trypan blue staining. Ho.33342 and PI double staining revealed that apoptosis as well as necrosis took place during glutamate treatment, and apoptosis was the main type of cell death. Oxidative stress played an important role in glutamate-induced cell damage but pretreatment with edaravone alleviated cell death. Results of western blot demonstrated that mechanisms underlying the toxicity of glutamate and the protection of edaravone were related to the PI3K pathway and Bcl-2 protein family.
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影响因子：
5
作者：
Amemiya, S;Kamiya, T;Katayama, Y
通讯作者：
Katayama, Y
影响因子：
16.2
作者：
ANKARCRONA, M;DYPBUKT, JM;NICOTERA, P
通讯作者：
NICOTERA, P
影响因子：
11.2
作者：
DAWSON, TM;DAWSON, VL;SNYDER, SH
通讯作者：
SNYDER, SH
影响因子：
2.9
作者：
JANSSEN, R;SCHWEITZER, L;JENSEN, KF
通讯作者：
JENSEN, KF
影响因子：
3.4
作者：
Liu, Wenwen;Fan, Zhaomin;Wang, Haibo
通讯作者：
Wang, Haibo