Micropilot: automation of fluorescence microscopy-based imaging for systems biology.
Micropilot: automation of fluorescence microscopy-based imaging for systems biology.
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DOI:
10.1038/nmeth.1558
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发表时间:
2011-03
期刊:
影响因子:
48
通讯作者:
Ellenberg, Jan
中科院分区:
文献类型:
--
作者:
Conrad, Christian;Wuensche, Annelie;Tan, Tze Heng;Bulkescher, Jutta;Sieckmann, Frank;Verissimo, Fatima;Edelstein, Arthur;Walter, Thomas;Liebel, Urban;Pepperkok, Rainer;Ellenberg, Jan
Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The ‘Micropilot’ software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.
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DOI:
10.1093/bioinformatics/btq046
发表时间:
2010-04-01
期刊:
Bioinformatics (Oxford, England)
影响因子:
--
作者:
Pau G;Fuchs F;Sklyar O;Boutros M;Huber W
通讯作者:
Huber W
影响因子:
64.8
作者:
通讯作者:
--
影响因子:
3.3
作者:
Hammond, AT;Glick, BS
通讯作者:
Glick, BS
影响因子:
--
作者:
Erfle, Holger;Neumann, Beate;Pepperkok, Rainer
通讯作者:
Pepperkok, Rainer
影响因子:
5.8
作者:
Raemoe, Pauli;Sacher, Raphael;Pelkmans, Lucas
通讯作者:
Pelkmans, Lucas