Micropilot: automation of fluorescence microscopy-based imaging for systems biology.

Micropilot: automation of fluorescence microscopy-based imaging for systems biology.
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DOI:
10.1038/nmeth.1558
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发表时间:
2011-03
期刊:
影响因子:
48
通讯作者:
Ellenberg, Jan
Ellenberg, Jan
中科院分区:
生物学1区
文献类型:
--
作者:
Conrad, Christian;Wuensche, Annelie;Tan, Tze Heng;Bulkescher, Jutta;Sieckmann, Frank;Verissimo, Fatima;Edelstein, Arthur;Walter, Thomas;Liebel, Urban;Pepperkok, Rainer;Ellenberg, Jan

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定量显微镜依赖于大量细胞的成像,但常常受到耗时的特定细胞手动选择的阻碍。 “Micropilot”软件自动检测感兴趣的细胞并启动复杂的成像实验,包括活细胞光漂白后的三维多色延时或荧光恢复。在三个独立的实验装置中,这使我们能够详细地统计分析生物过程,因此是系统生物学的强大工具。
Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The ‘Micropilot’ software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.
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