Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls.
Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls.
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水稻对飞虱的抗性对 OsEXO70H3 调节 SAMSL 排泄和细胞壁木质素沉积的必要性
DOI：
10.1111/nph.18012
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发表时间：
2022-05
期刊：
影响因子：
9.4
通讯作者：
He, Guangcun
中科院分区：
文献类型：
--
作者：
Wu, Di;Guo, Jianping;Zhang, Qian;Shi, Shaojie;Guan, Wei;Zhou, Cong;Chen, Rongzhi;Du, Bo;Zhu, Lili;He, Guangcun
抗稻飞虱基因Bph 6编码与OsEXO 70 E1相互作用的蛋白。EXO 70形成水稻中的旁系同源物家族。我们假设EXO 70依赖的运输途径影响抗性相关蛋白的分泌，从而影响植物对稻飞虱的抗性。本文进一步探讨了EXO 70成员在水稻抗稻飞虱中的功能。我们使用酵母双杂交和免疫共沉淀试验来鉴定在Bph 6介导的稻飞虱抗性中发挥作用的蛋白质。通过基因转化、植物抗性鉴定、昆虫表现、细胞分泌观察和细胞壁成分分析，对所鉴定的蛋白质的功能进行了表征。我们发现另一个EXO 70成员OsEXO 70 H3与Bph 6相互作用，并在细胞分泌和Bph 6介导的抗稻飞虱中发挥作用。我们进一步发现OsEXO 70 H3与S-腺苷甲硫氨酸合成酶样蛋白（SAMSL）相互作用，并增加SAMSL向细胞外的递送。OsEXO 70 H3和SAMSL的功能受损降低了水稻植株的木质素含量和稻飞虱抗性水平。我们的研究结果表明OsEXO 70 H3可以招募SAMSL并帮助其分泌到质外体，在那里它可能参与木质素在细胞壁中的沉积，从而有助于水稻对稻飞虱的抗性。
The planthopper resistance gene Bph6 encodes a protein that interacts with OsEXO70E1. EXO70 forms a family of paralogues in rice. We hypothesized that the EXO70‐dependent trafficking pathway affects the excretion of resistance‐related proteins, thus impacting plant resistance to planthoppers. Here, we further explored the function of EXO70 members in rice resistance against planthoppers. We used the yeast two‐hybrid and co‐immunoprecipitation assays to identify proteins that play roles in Bph6‐mediated planthopper resistance. The functions of the identified proteins were characterized via gene transformation, plant resistance evaluation, insect performance, cell excretion observation and cell wall component analyses. We discovered that another EXO70 member, OsEXO70H3, interacted with BPH6 and functioned in cell excretion and in Bph6‐mediated planthopper resistance. We further found that OsEXO70H3 interacted with an S‐adenosylmethionine synthetase‐like protein (SAMSL) and increased the delivery of SAMSL outside the cells. The functional impairment of OsEXO70H3 and SAMSL reduced the lignin content and the planthopper resistance level of rice plants. Our results suggest that OsEXO70H3 may recruit SAMSL and help its excretion to the apoplast where it may be involved in lignin deposition in cell walls, thus contributing to rice resistance to planthoppers.
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