Derivative Technology of DNA Barcoding (Nucleotide Signature and SNP Double Peak Methods) Detects Adulterants and Substitution in Chinese Patent Medicines.
Derivative Technology of DNA Barcoding (Nucleotide Signature and SNP Double Peak Methods) Detects Adulterants and Substitution in Chinese Patent Medicines.
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DNA条形码衍生技术（核苷酸特征和SNP双峰法）检测中成药中的掺假和替代
DOI：
10.1038/s41598-017-05892-y
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发表时间：
2017-07-19
影响因子：
4.6
通讯作者：
Newmaster SG
中科院分区：
文献类型：
--
作者：
Gao Z;Liu Y;Wang X;Song J;Chen S;Ragupathy S;Han J;Newmaster SG
金银花在我国已被用于生产上百种中成药。出于经济动机的掺假已被记录在案，导致市场不稳定和消费者信心下降。ITS2已用于药材的鉴别，但不适合植物提取物和复杂中成药的鉴别。因此，使用短条形码来识别已处理的CPM将是有利可图的。根据Eurobacterium的ITS 2区的独特结构域，设计了一个34 bp的核苷酸序列（5′ CTAGCGGTGGTCGTACGATAGCCAATGCATGAGT 3′）。粉末状的芍药花和芍药花的混合物在预期的SNP（单核苷酸多态性）位置处产生双峰，其中峰的高度大致指示混合粉末中的物种比例。随后，我们测试了20种提取物和47种标记为含有一些种类的黑木耳的中成药。结果表明，只有17%的提取物和22%的中成药是真实的，其余的都存在替代或掺假，7%的提取物中同时含有杜仲和红花两种掺假物。本研究所建立的方法将拓宽DNA条形码在天然保健品质量保证中的应用。
Lonicerae japonicae Flos has been used to produce hundred kinds of Chinese patent medicines (CPMs) in China. Economically motivated adulterants have been documented, leading to market instability and a decline in consumer confidence. ITS2 has been used to identify raw medicinal materials, but it’s not suitable for the identification of botanical extracts and complex CPMs. Therefore, a short barcode for the identification of processed CPMs would be profitable. A 34 bp nucleotide signature (5′ CTAGCGGTGGTCGTACGATAGCCAATGCATGAGT 3′) was developed derived from ITS2 region of Eucommiae Folium based on unique motifs. Mixtures of powdered Lonicerae japonicae Flos and Lonicerae Flos resulted in double peaks at the expected SNP (Single Nucleotide Polymorphisms) positions, of which the height of the peaks were roughly indicative of the species’ ratio in the mixed powder. Subsequently we tested 20 extracts and 47 CPMs labelled as containing some species ofLonicera. The results revealed only 17% of the extracts and 22% of the CPMs were authentic, others exist substitution or adulterant; 7% were shown to contain both of two adulterants Eucommiae Folium and Lonicerae Flos. The methods developed in this study will widely broaden the application of DNA barcode in quality assurance of natural health products.
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影响因子：
5.6
作者：
Liu Y;Wang X;Wang L;Chen X;Pang X;Han J
通讯作者：
Han J
影响因子：
2.1
作者：
Gaudiano, Maria Cristina;Manna, Livia;Valvo, Luisa
通讯作者：
Valvo, Luisa
影响因子：
9.3
作者：
Newmaster SG;Grguric M;Shanmughanandhan D;Ramalingam S;Ragupathy S
通讯作者：
Ragupathy S
影响因子：
3.7
作者：
Kiebala M;Polesskaya O;Yao Z;Perry SW;Maggirwar SB
通讯作者：
Maggirwar SB
影响因子：
4.2
作者：
de Boer, Hugo J.;Ichim, Mihael C.;Newmaster, Steven G.
通讯作者：
Newmaster, Steven G.