Recruitment of resting vesicles into recycling pools supports NMDA receptor-dependent synaptic potentiation in cultured hippocampal neurons.
Recruitment of resting vesicles into recycling pools supports NMDA receptor-dependent synaptic potentiation in cultured hippocampal neurons.
复制标题
将静止囊泡募集到回收池中支持培养的海马神经元中NMDA受体依赖性突触增强。
DOI：
10.1113/jphysiol.2011.226688
复制
发表时间：
2012-04-01
期刊：
影响因子：
--
通讯作者：
Staras K
中科院分区：
文献类型：
--
作者：
Ratnayaka A;Marra V;Bush D;Burden JJ;Branco T;Staras K
中枢神经系统中的大多数突触前末梢的特征在于两个功能不同的囊泡群体：一个再循环池，其通过囊泡胞吐作用支持动作电位驱动的神经递质释放，和一个静息池。这两个池的相对比例在单个突触之间变化很大，促使人们猜测它们的特定关系，以及静息池的可能功能。使用荧光成像的FM-苯乙烯基染料和synaptophysinI-pHluorin（sypHy），以及相关的电子显微镜方法，我们在这里表明，在大鼠海马神经元突触强度的赫布可塑性依赖的变化可以增加回收池分数在个别突触末梢的静息池的费用。这一募集过程依赖于NMDA受体激活、一氧化氮信号传导和钙调神经磷酸酶，并伴随着单个末端神经递质释放概率的增加。肌动蛋白介导的突触间囊泡交换的封锁并不能阻止再循环池的扩大，表明囊泡募集是突触内的。我们建议，休息池囊泡的转换功能回收池提供了一个快速的机制，实现突触前效力的持久变化。
Most presynaptic terminals in the central nervous system are characterized by two functionally distinct vesicle populations: a recycling pool, which supports action potential-driven neurotransmitter release via vesicle exocytosis, and a resting pool. The relative proportions of these two pools are highly variable between individual synapses, prompting speculation on their specific relationship, and on the possible functions of the resting pool. Using fluorescence imaging of FM-styryl dyes and synaptophysinI-pHluorin (sypHy) as well as correlative electron microscopy approaches, we show here that Hebbian plasticity-dependent changes in synaptic strength in rat hippocampal neurons can increase the recycling pool fraction at the expense of the resting pool in individual synaptic terminals. This recruitment process depends on NMDA-receptor activation, nitric oxide signalling and calcineurin and is accompanied by an increase in the probability of neurotransmitter release at individual terminals. Blockade of actin-mediated intersynaptic vesicle exchange does not prevent recycling pool expansion demonstrating that vesicle recruitment is intrasynaptic. We propose that the conversion of resting pool vesicles to the functionally recycling pool provides a rapid mechanism to implement long-lasting changes in presynaptic efficacy.
登录
查看更多内容
影响因子：
25
作者：
Fredj, Naila Ben;Burrone, Juan
通讯作者：
Burrone, Juan
影响因子：
5.3
作者：
Fisher-Lavie, Arava;Zeidan, Adel;Ziv, Noam E.
通讯作者：
Ziv, Noam E.
影响因子：
16.2
作者：
Kim, Sung Hyun;Ryan, Timothy A.
通讯作者：
Ryan, Timothy A.
影响因子：
56.9
作者：
BETZ, WJ;BEWICK, GS
通讯作者：
BEWICK, GS
DOI：
10.1007/s11068-008-9030-y
发表时间：
2008-08-01
期刊：
BRAIN CELL BIOLOGY
影响因子：
--
作者：
Fernandez-Alfonso, Tomas;Ryan, Timothy A.
通讯作者：
Ryan, Timothy A.