Influence of animal husbandry practices on void spot assay outcomes in C57BL/6J male mice.
Influence of animal husbandry practices on void spot assay outcomes in C57BL/6J male mice.
复制标题
畜牧业对C57BL/6J雄性小鼠无效现场测定结果的影响。
DOI：
10.1002/nau.22692
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发表时间：
2016-02
影响因子：
2
通讯作者：
Vezina, Chad M.
中科院分区：
文献类型：
--
作者：
Keil, Kimberly P.;Abler, Lisa L.;Altmann, Helene M.;Bushman, Wade;Marker, Paul C.;Li, Lingjun;Ricke, William A.;Bjorling, Dale E.;Vezina, Chad M.
小鼠越来越多地被用作模型来研究具有下尿路症状（LUTS）的人类经历的泌尿功能障碍的方面。用于检查排尿功能的一种方法是自发性空斑测定。本研究的目的是表征和确定可能混淆雄性C57 Bl/6 J小鼠自发空斑试验结果的动物饲养条件。将小鼠置于内衬有滤纸的笼中4小时，并用UV透照观察尿液。对排尿参数包括尿点数量、尿点大小、总尿面积、原发性排尿面积、角落和中心排尿进行定量。成年雄性小鼠随着年龄的增长排尿更频繁，并且亚群（5-10%）在6-9周龄时显示出频繁的斑点模式。与单笼饲养相比，断奶至组笼饲养（每笼4-6只）的雄性小鼠的排尿没有显著差异，并且当它们用作育种者时也没有改变。在将组饲养的成年雄性动物转移到单密度笼中后，排尿发生变化，这降低了总尿液面积。连续3天重复测定雄性动物排尿行为，到监测第3天，原发性排尿面积增加，并显示排尿行为受常规笼舍更换和一天中时间的影响。这些结果共同确定了影响自发排尿点试验中雄性排尿行为的饲养和饲养实践，并将为用雄性C57 Bl/6 J小鼠进行的排尿行为分析提供信息。
Mice are increasingly being used as models to investigate aspects of urinary dysfunction that humans with lower urinary tract symptoms (LUTS) experience. One method used to examine voiding function is the spontaneous void spot assay. The purpose of this study was to characterize and identify animal husbandry conditions that might confound results of the spontaneous void spot assay in male C57Bl/6J mice. Mice were placed in cages lined with filter paper for four hours and urine was visualized with UV transillumination. Voiding parameters including urine spot number, spot size, total urine area, primary void area, corner and center voiding were quantified. Adult male mice void more frequently with advancing age and a subpopulation (5–10%) display a frequent spotting pattern at 6–9 weeks of age. Voiding was not significantly different in male mice weaned to group housing (4–6 per cage) versus single housing, and was not altered when they were used as breeders. Voiding was changed upon transferring group housed adult males to single density cages, which decreased total urine area. Repeated assays of male voiding behavior over three consecutive days increased primary void area by the third day of monitoring and revealed that voiding behavior is impacted by routine cage changes and time of day. Together these results identify housing and husbandry practices that influence male voiding behaviors in the spontaneous void spot assay and will inform voiding behavior analyses conducted with male C57Bl/6J mice.
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影响因子：
6.6
作者：
Hodges, Steve J.;Zhou, Ge;Christ, George J.
通讯作者：
Christ, George J.
影响因子：
3.5
作者：
KIMURA, T;HAGIWARA, Y
通讯作者：
HAGIWARA, Y
影响因子：
2.7
作者：
Mucignat-Caretta, Carla;Cavaggioni, Andrea;Gabai, Gianfranco
通讯作者：
Gabai, Gianfranco
DOI：
10.1098/rspb.2000.1057
发表时间：
2000-04-07
影响因子：
4.7
作者：
Nevison, CM;Barnard, CJ;Hurst, JL
通讯作者：
Hurst, JL
影响因子：
56.9
作者：
RALLS, K
通讯作者：
RALLS, K