Effect of Obesity or Metabolic Syndrome and Diabetes on Osseointegration of Dental Implants in a Miniature Swine Model: A Pilot Study.
Effect of Obesity or Metabolic Syndrome and Diabetes on Osseointegration of Dental Implants in a Miniature Swine Model: A Pilot Study.
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DOI：
10.1016/j.joms.2018.02.021
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Dard M
中科院分区：
文献类型：
--
作者：
Coelho PG;Pippenger B;Tovar N;Koopmans SJ;Plana NM;Graves DT;Engebretson S;van Beusekom HMM;Oliveira PGFP;Dard M
肥胖/代谢综合征（O/MS）和糖尿病（DM）的患病率不断增加，仍然是全球健康问题。缺乏临床相关的和实用的翻译模型，模仿这些条件的人类特征。本研究旨在证明在哥廷根小型猪模型中诱导稳定型肥胖/代谢综合征（O/MS）和2型糖尿病（DM）的概念证据，并验证这些疾病调整的哥廷根小型猪模型作为牙种植体测试的愈合受损模型。将9只小型猪分为3组：对照组（正常饮食）、肥胖组（自助餐饮食）和糖尿病组（自助餐饮食+链脲佐菌素），然后放置牙种植体。记录每个研究组的炎症标志物，包括TNF-α、C反应蛋白和皮质醇。进行了移除扭矩和组织形态学分析（骨-种植体接触（BIC）和骨面积分数占用（BAFO））。与对照组相比，O/MS猪的血浆C-反应蛋白（p<0.05）和皮质醇（p<0.09）浓度平均增加2倍;与对照组相比，DM猪的血浆TNF-α（p<0.05）平均增加40倍，皮质醇（p<0.05）浓度平均增加2倍。确定了O/MS和DM对植入物的影响。对照组（200 Ncm）的界面失效扭矩最高，O/MS组（90 Ncm）和DM组（60 Ncm）的界面失效扭矩显著较低（p<0.01）。对照组种植体周围的骨形成显著大于O/MS和DM组（p<0.02）。O/MS和DM小型猪均表达类人疾病表型，并且均呈现牙种植体周围的骨愈合障碍。2型糖尿病和肥胖/代谢综合征对种植体周围骨形成的影响无显著差异，这证明有必要进一步研究肥胖/代谢综合征的影响。
The increasing prevalence of obesity/metabolic syndrome (O/MS) and diabetes mellitus (DM) remains a global health concern. Clinically relevant and practical translational models mimicking human characteristics of these conditions are lacking. This study aimed to demonstrate proof of concept of the induction of stable obesity/metabolic syndrome (O/MS) and type-2 diabetes mellitus (DM) in a Göttingen minipig model and validate both of these disease-adjusted Göttingen mini-pig models as impaired healing models for the testing of dental implants. 9 minipigs were split into 3 groups: control (normal diet), obese (cafeteria diet) and diabetic (cafeteria diet + Streptozotocin), and followed by placement of dental implants. Inflammatory markers including TNF-α, C-reactive protein, and cortisol were recorded for each study group. Removal torque and histomorphometric analysis (bone to implant contact (BIC) and bone area fraction occupancy (BAFO)) were performed. O/MS pigs showed, on average, a 2-fold increase in plasma C-reactive protein (p<0.05) and cortisol (p<0.09) concentrations compared to controls; DM pigs showed, on average, a 40-fold increase in plasma TNF-α (p<0.05) and a 2-fold increase in cortisol (p<0.05) concentrations compared to controls. The impact of O/MS and DM on implants was determined. Torque to interface failure was highest in control (200 Ncm), and significantly lower in O/MS (90 Ncm) and DM (60 Ncm) groups (p<0.01). Bone formation around implants was significantly greater in control than O/MS and DM (p<0.02). Both O/MS and DM minipigs express human-like disease phenotype and both presented bone healing impairment around dental implants. No significant difference between type-2 diabetes and obesity/metabolic syndrome on bone formation around implants provides evidence that further investigation of the impact of obesity/metabolic syndrome is warranted.
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影响因子：
2.2
作者：
GABEL, H;BITTERSUERMANN, H;BRYNGER, H
通讯作者：
BRYNGER, H
影响因子：
2.2
作者：
Friedmann, Anton;Friedmann, Asisa;Dard, Michel
通讯作者：
Dard, Michel
影响因子：
4.3
作者：
Iyama, S;Takeshita, F;Tanaka, T
通讯作者：
Tanaka, T
影响因子：
5.6
作者：
Doucette CR;Horowitz MC;Berry R;MacDougald OA;Anunciado-Koza R;Koza RA;Rosen CJ
通讯作者：
Rosen CJ
影响因子：
2.6
作者：
Cao JJ
通讯作者：
Cao JJ