Interleukin 10 and interferon gamma regulation of experimental Trypanosoma cruzi infection.
Interleukin 10 and interferon gamma regulation of experimental Trypanosoma cruzi infection.
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DOI：
10.1084/jem.175.1.169
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发表时间：
1992-01-01
期刊：
影响因子：
--
通讯作者：
Reed SG
中科院分区：
文献类型：
--
作者：
Silva JS;Morrissey PJ;Grabstein KH;Mohler KM;Anderson D;Reed SG
进行研究以确定白细胞介素10（IL-10）（一种显示抑制干扰素γ（IFN-γ）产生的细胞因子）是否参与小鼠中的克氏锥虫感染。外源性IFN-γ保护小鼠免受T.克鲁兹此外，耐药B6 D2小鼠产生致命的T.当用中和性抗IFN-γ单克隆抗体（mAb）治疗时，因此，内源性以及外源性IFN-γ在介导对该寄生虫的抗性中是重要的。因为T. Cruzi敏感（B6）和耐药（B6 D2）小鼠品系在急性感染期间产生IFN-γ，我们寻找可干扰IFN-γ介导的对T.克鲁兹我们发现，IL-10特异性mRNA在急性T细胞白血病小鼠脾脏中产生。克鲁兹感染。此外，感染B6小鼠的脾细胞培养物上清液，以及在较小程度上B6 D2小鼠的脾细胞培养物上清液，产生IFN-γ产生的抑制剂。该抑制剂被抗IL-10 mAb中和。这些实验证实了T.克氏感染在进一步的体外研究中，显示IL-10阻断IFN-γ抑制T细胞内复制的能力。小鼠腹腔巨噬细胞中的Cruzi。因此，除了其已知的抑制IFN-γ产生的能力之外，IL-10（细胞因子合成抑制因子）也可以抑制IFN-γ的作用。这些实验表明，原生动物寄生虫感染期间会产生IL-10，并表明这种细胞因子在介导对急性疾病的易感性方面具有调节作用。
Studies were undertaken to determine whether interleukin 10, (IL-10) a cytokine shown to inhibit interferon gamma (IFN-gamma) production, was involved in Trypanosoma cruzi infections in mice. Exogenous IFN-gamma protects mice from fatal infection with T. cruzi. Furthermore, resistant B6D2 mice developed fatal T. cruzi infections when treated with neutralizing anti-IFN-gamma monoclonal antibody (mAb). Thus, endogenous as well as exogenous IFN-gamma is important in mediating resistance to this parasite. Because both T. cruzi-susceptible (B6) and -resistant (B6D2) mouse strains produced IFN-gamma during acute infection, we looked for the concomitant production of mediators that could interfere with IFN-gamma-mediated resistance to T. cruzi. We found that IL-10-specific mRNA was produced in the spleens of mice with acute T. cruzi infections. In addition, spleen cell culture supernatants from infected B6 mice, and to a lesser extent B6D2 mice, elaborated an inhibitor(s) of IFN-gamma production. This inhibitor(s) was neutralized by anti-IL-10 mAb. These experiments demonstrated the production of biologically active IL-10 during T. cruzi infection. In further studies in vitro, it was shown that IL-10 blocked the ability of IFN-gamma to inhibit the intracellular replication of T. cruzi in mouse peritoneal macrophages. Thus, in addition to its known ability to inhibit the production of IFN-gamma, IL-10 (cytokine synthesis inhibitory factor), may also inhibit the effects of IFN-gamma. These experiments demonstrate that IL-10 is produced during infection with a protozoan parasite and suggest a regulatory role for this cytokine in the mediation of susceptibility to acute disease.
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DOI：
10.1084/jem.169.1.59
发表时间：
1989-01-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Heinzel FP;Sadick MD;Holaday BJ;Coffman RL;Locksley RM
通讯作者：
Locksley RM
影响因子：
3.7
作者：
ROTTENBERG, M;LINDQVIST, C;ORN, A
通讯作者：
ORN, A
DOI：
10.1084/jem.170.6.2081
发表时间：
1989-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Fiorentino DF;Bond MW;Mosmann TR
通讯作者：
Mosmann TR
影响因子：
56.9
作者：
MOORE, KW;VIEIRA, P;MOSMANN, TR
通讯作者：
MOSMANN, TR
DOI：
10.1084/jem.166.5.1229
发表时间：
1987-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Cherwinski HM;Schumacher JH;Brown KD;Mosmann TR
通讯作者：
Mosmann TR