The Mechanism of Action of Pterostilbene in Xinjiang Wine Grape Against the Growth of Geotrichum citri-aurantii
The Mechanism of Action of Pterostilbene in Xinjiang Wine Grape Against the Growth of Geotrichum citri-aurantii
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新疆酿酒葡萄中紫檀芪抑制柑橘地霉生长的作用机制
DOI：
10.1080/08905436.2016.1198705
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发表时间：
2016-07
影响因子：
1.8
通讯作者：
Pan Yuanjiang
中科院分区：
文献类型：
--
作者：
Kong Qingjun;Ren Xueyan;Tu LiDan;Zheng Xiaodong;Wang Zhidong;Pan Yuanjiang
摘要紫檀芪对柑桔地霉节孢子萌发有较强的抑制作用。利用光学显微镜、扫描电镜等手段对G.用紫檀芪处理的柑橘显著改变。一些细胞异常或溶解。为了进一步阐明作用的分子机制，我们研究了紫檀芪对G.柑橘属真菌细胞结果表明，G.柑桔属真菌细胞被紫檀芪降解。并对3个真菌凋亡相关基因进行半定量RT-PCR检测。结果表明，紫檀芪能上调RLM 1、RTG 1和RTG 3相关基因的表达，其中前两个基因的表达显著上调。
ABSTRACT Pterostilbene controlled the growth of Geotrichum citri-aurantii effectively through experiments of arthroconidium germination on agar plate, which displayed a strong antifungal activity. Observations using light microscope, scanning electron showed that the morphology and inner structure of the G. citri-aurantii treated with the pterostilbene changed notably. Some of the cells were abnormal or lysed. In order to further clarify the molecular mechanism of action, we examined the binding properties exerted by the pterostilbene on DNA and RNA of G. citri-aurantii fungal cells. Results showed that DNA and RNA of the G. citri-aurantii fungal cells were degraded by the pterostilbene. Moreover, three differentially expressed apoptosis genes of fungi were further examined by semi-quantitative RT-PCR. The results indicated that pterostilbene could cause the expression of up-regulated genes involved in RLM1, RTG1, and RTG3, of which the former two genes were significantly up-regulated.
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DOI：
10.3390/molecules19067480
发表时间：
2014-06-06
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Zernova OV;Lygin AV;Pawlowski ML;Hill CB;Hartman GL;Widholm JM;Lozovaya VV
通讯作者：
Lozovaya VV
影响因子：
6.1
作者：
Furlanetto, Valentina;Zagotto, Giuseppe;Gatto, Barbara
通讯作者：
Gatto, Barbara
影响因子：
3.9
作者：
Renato X Santos;S. Cardoso;Sónia Silva;S. Correia;C. Carvalho;J. Crisóstomo;L. Rodrigues;C. Amaral;T. Louro;P. Matafome;Maria S. Santos;T. Proença;Ana I. Duarte;R. Seiça;Paula I. Moreira
通讯作者：
Renato X Santos;S. Cardoso;Sónia Silva;S. Correia;C. Carvalho;J. Crisóstomo;L. Rodrigues;C. Amaral;T. Louro;P. Matafome;Maria S. Santos;T. Proença;Ana I. Duarte;R. Seiça;Paula I. Moreira
影响因子：
3.9
作者：
Chi-Yao Hou;Y.‐S. Lin;Y. T. Wang;C-M Jiang;K. -. Lin;M. Wu
通讯作者：
Chi-Yao Hou;Y.‐S. Lin;Y. T. Wang;C-M Jiang;K. -. Lin;M. Wu
影响因子：
6.1
作者：
Schulze, K;Schreiber, L;Szankowski, I
通讯作者：
Szankowski, I