Ion mobility mass spectrometry for the study of mycobacterial mycolic acids.
Ion mobility mass spectrometry for the study of mycobacterial mycolic acids.
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DOI：
10.1038/s41598-023-37641-9
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发表时间：
2023-06-27
影响因子：
4.6
通讯作者：
Larrouy-Maumus, Gerald
中科院分区：
文献类型：
--
作者：
Liu, Yi;Kaffah, Nadhira;Pandor, Sufyan;Sartain, Mark J.;Larrouy-Maumus, Gerald
脂质是结构高度多样化的分子，参与多种生物过程。脂质的参与在分枝杆菌中甚至更明显，包括人类病原体结核分枝杆菌，其在细胞包膜中产生高度复杂和多样化的脂质组。这些脂质包括分枝菌酸，分枝菌酸是自然界中最长的脂肪酸之一，可以含有多达90个碳原子。分枝菌酸普遍存在于分枝杆菌中，是α分支和β羟基化脂质。离散修饰，如α、α '、环氧基、甲氧基、酮基和羧基，在物种水平上表征分枝菌酸。在这里，我们使用高精度离子迁移率质谱法建立了一个数据库，其中包括206个来自严格的人类病原体M的分枝菌酸的质量分辨碰撞截面（CCS）。结核病的机会菌株M. M.P. marinum和M. avium和非致病性M.恶臭在分枝杆菌种属之间可以观察到分枝菌酸谱之间的主要差异。酰基尾长和修饰是决定CCS大小的主要结构描述符。作为研究人员的资源，这项工作提供了一个详细的目录的质量分辨碰撞横截面的分枝菌酸沿着的工作流程，以生成和分析生成的数据集。
Lipids are highly structurally diverse molecules involved in a wide variety of biological processes. The involvement of lipids is even more pronounced in mycobacteria, including the human pathogen Mycobacterium tuberculosis, which produces a highly complex and diverse set of lipids in the cell envelope. These lipids include mycolic acids, which are among the longest fatty acids in nature and can contain up to 90 carbon atoms. Mycolic acids are ubiquitously found in mycobacteria and are alpha branched and beta hydroxylated lipids. Discrete modifications, such as alpha, alpha’, epoxy, methoxy, keto, and carboxy, characterize mycolic acids at the species level. Here, we used high precision ion mobility-mass spectrometry to build a database including 206 mass-resolved collision cross sections (CCSs) of mycolic acids originating from the strict human pathogen M. tuberculosis, the opportunistic strains M. abscessus, M. marinum and M. avium, and the nonpathogenic strain M. smegmatis. Primary differences between the mycolic acid profiles could be observed between mycobacterial species. Acyl tail length and modifications were the primary structural descriptors determining CCS magnitude. As a resource for researchers, this work provides a detailed catalogue of the mass-resolved collision cross sections for mycolic acids along with a workflow to generate and analyse the dataset generated.
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影响因子：
5.4
作者：
Jackson, Mary
通讯作者：
Jackson, Mary
影响因子：
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作者：
Barkan D;Liu Z;Sacchettini JC;Glickman MS
通讯作者：
Glickman MS
影响因子：
2.8
作者：
MINNIKIN, DE;MINNIKIN, SM;MAGNUSSON, M
通讯作者：
MAGNUSSON, M
影响因子：
4.4
作者：
Dkhar, Hedwin Kitdorlang;Nanduri, Ravikanth;Gupta, Pawan
通讯作者：
Gupta, Pawan
分枝菌酸：破译并瞄准结核杆菌的致命弱点。
影响因子：
3.6
作者：
Nataraj V;Varela C;Javid A;Singh A;Besra GS;Bhatt A
通讯作者：
Bhatt A