Simultaneous determination of paraquat and diquat in human plasma by HPLC-DAD: Its application in acute poisoning patients induced by these two herbicides.-猫眼课题宝

HPLC-DAD同时测定人血浆中百草枯和甘菊：在两种除草剂急性中毒患者中的应用。

DOI：

10.1002/jcla.23669

发表时间：

2021-03

期刊：

Journal of clinical laboratory analysis

影响因子：

2.7

通讯作者：

中科院分区：

医学4区

文献类型：

作者：

Yuan G;Li R;Zhao Q;Kong X;Wang Y;Wang X;Guo R

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957985/

百草枯和双菊枯在许多国家的农业生产中被广泛使用，对人体有很大的毒性。在市场上出售的一些双菊枯溶液中可以检测到百草枯。血药浓度是临床诊断百草枯或百草枯中毒的重要指标。因此，建立一种同时测定人血浆中百草枯和甘菊枯的方法具有十分重要的意义。建立HPLC - DAD同时测定人血浆中百草枯和双氰菊酯的方法，并应用于急性百草枯和双氰菊酯中毒患者。HPLC - DAD法同时测定百草枯和地菊。血浆经Waters OASIS®色谱柱处理后，采用Thermo Hypersil GOLD （250 × 4.6 mm, 5 μm）色谱柱分离，流动相为75 mmol/L正丁酸钠（含0.1 mol/L磷酸，pH 3.0）和乙腈（87:13,v:v），流速为1.0 mL/min。全波长扫描范围为200 ~ 400 nm，百草枯和迪奎特的检测波长分别为257nm和310nm。采用所建立的方法对120例和30例百草枯中毒患者的血浆标本进行分析。百草枯和双菊枯的标准曲线范围为0.05 ~ 20 μg/mL，百草枯的下限精密度为16.49%，要求精密度小于20%。其他浓度的精密度均小于14.14%。百草枯和双菊枯的回收率分别为95.38%‐103.97%和94.79%‐98.40%。结果表明，百草枯和双菊枯在不同的贮藏条件下均保持稳定。采用所建立的方法对120例百草枯中毒患者和30例地奎特中毒患者的血浆样本进行了检测。百草枯血药浓度范围为0.10 ~ 20.62 μg/mL，平均为3.61 μg/mL；地喹特血药浓度范围为0 ~ 26.59 μg/mL，平均为2.00 μg/mL。在疑似中毒样品中，5份样品同时检测到双氰菊酯和百草枯，2份样品仅检测到百草枯，未检测到双氰菊酯。本研究建立的HPLC - DAD方法具有高通量、高灵敏度、操作简单、线性范围宽等特点。该方法可用于急性中毒患者中百草枯和迪奎特的筛选分析和定量检测，为这两种除草剂中毒患者的治疗和预后提供依据。用所建立的方法测定了一例疑似白藜芦醇中毒患者血浆中的百草枯和白藜芦醇。百草枯和双菊枯的滞留时间分别为7.646 min和8.771 min。

Paraquat and diquat are widely used in agricultural production in many countries, which are very toxic to human beings. Paraquat can be detected in some diquat solution sold in the market. The blood concentration of paraquat or diquat is an important indicator for clinical diagnosis of paraquat or diquat poisoning. So, it is very meaningful to develop a method for simultaneous determination of paraquat and diquat in human plasma. To develop and validate a HPLC‐DAD method for simultaneous determination of paraquat and diquat in human plasma and to apply it in the acute poisoning patients by these two herbicides. Paraquat and diquat were simultaneously determined by HPLC‐DAD. The plasma was treated using Waters OASIS® Column and then separated on a Thermo Hypersil GOLD (250 × 4.6 mm, 5 μm) Column with the mobile phase consisted of 75 mmol/L sodium heptane sulfonate (containing 0.1 mol/L phosphoric acid, pH 3.0) and acetonitrile (87:13, v:v) at a flow rate of 1.0 mL/min. The full‐wavelength scanning was 200‐400 nm, and the detection wavelength of paraquat and diquat was 257nm and 310nm, respectively. 120 and 30 plasma samples from patients with paraquat and diquat poisoning were collected and analyzed by the established method. The standard curve for paraquat and diquat ranged from 0.05 to 20 μg/mL, and the precision of LLOQ for paraquat was 16.49%, which was required to be less than 20%. The precision of other concentrations was less than 14.14%. The recovery of paraquat and diquat was 95.38%‐103.97% and 94.79%‐98.40%, respectively. The results showed that paraquat and diquat were stable under various storage conditions. 120 plasma samples of paraquat poisoning patients and 30 plasma samples of diquat poisoning patients were determined by the established method. The blood concentration of paraquat ranged from 0.10 to 20.62 μg/mL, with an average of 3.61 μg/mL, while for diquat, the concentration ranged from 0 to 26.59 μg/mL, with an average of 2.00 μg/mL. Among the diquat suspected poisoning samples, 5 samples were detected not only diquat but also paraquat, and 2 samples were detected only paraquat, no diquat. The HPLC‐DAD method established in this study was high throughput, high sensitivity, simple operation, and wide linear ranges. It can be used for the screening analysis and quantitative detection of paraquat and diquat in acute poisoning patients, which can provide basis for the treatment and prognosis of these two herbicides poisoning patients. Both paraquat and diquat were determined in a suspected diquat poisoning patient plasma by the established method. The retention time of paraquat and diquat was 7.646 min and 8.771 min.