Nestin expression in osteosarcomas and derivation of nestin/CD133 positive osteosarcoma cell lines.
Nestin expression in osteosarcomas and derivation of nestin/CD133 positive osteosarcoma cell lines.
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DOI：
10.1186/1471-2407-8-300
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发表时间：
2008-10-16
期刊：
影响因子：
3.8
通讯作者：
Sterba J
中科院分区：
文献类型：
--
作者：
Veselska R;Hermanova M;Loja T;Chlapek P;Zambo I;Vesely K;Zitterbart K;Sterba J
巢蛋白最初被鉴定为VI类中间丝蛋白，其在发育期间在哺乳动物CNS的干细胞和祖细胞中表达。这种蛋白质在成年生物体中被其他中间丝蛋白取代;然而，巢蛋白可能在某些病理条件下重新表达，如缺血，炎症，脑损伤和肿瘤转化。巢蛋白在多种肿瘤中均有表达，尤其是中枢神经系统肿瘤。巢蛋白和CD 133表面分子的共表达被认为是神经源性肿瘤中癌症干细胞的标志物。我们的工作旨在详细研究巢蛋白在骨肉瘤和骨肉瘤衍生细胞系中的表达。采用免疫检测方法，我们检测了18例骨肉瘤患者的肿瘤组织样本中的巢蛋白。我们还成功地从4例患者的肿瘤组织中建立了永久性细胞系，并对这些细胞系进行了巢蛋白和CD 133的免疫检测。巢蛋白阳性肿瘤细胞在所有检查的骨肉瘤中均被免疫化学检测到，但这些细胞的阳性染色比例以及染色强度各不相同。巢蛋白阳性细胞很少观察到2个肿瘤样本，其余16个肿瘤样本显示不同的巢蛋白表达模式，从非常零星的发生与强阳性染色的细胞压倒性的比例。三个已建立的骨肉瘤细胞系被证明是巢蛋白阳性，只有一个细胞系显示没有巢蛋白的表达，这一发现对应于罕见的发生巢蛋白阳性细胞在相应的肿瘤样本。其中3株骨肉瘤细胞系为内斯+/CD 133+。我们的研究结果代表了巢蛋白在骨肉瘤中表达的第一个证据，并表明在这些肿瘤中可能存在干细胞样表型细胞。
Nestin was originally identified as a class VI intermediate filament protein that is expressed in stem cells and progenitor cells in the mammalian CNS during development. This protein is replaced in the adult organism by other intermediate filament proteins; however, nestin may be re-expressed under certain pathological conditions such as ischemia, inflammation, brain injury, and neoplastic transformation. Nestin has been detected in many kinds of tumors, especially in tumors derived from the CNS. Co-expression of nestin and the CD133 surface molecule is considered to be a marker for cancer stem cells in neurogenic tumors. Our work was aimed at a detailed study of nestin expression in osteosarcomas and osteosarcoma-derived cell lines. Using immunodetection methods, we examined nestin in tumor tissue samples from 18 patients with osteosarcomas. We also successfully established permanent cell lines from the tumor tissue of 4 patients and immunodetection of nestin and CD133 was performed on these cell lines. Nestin-positive tumor cells were immunohistochemically detected in all of the examined osteosarcomas, but the proportion of these cells that were positively stained as well as the intensity of staining varied. Nestin-positive cells were rarely observed in 2 tumor samples, and the remaining 16 tumor samples showed various nestin expression patterns ranging from very sporadic occurrence to an overwhelming proportion of cells with strong positive staining. Three of the established osteosarcoma cell lines were demonstrated to be nestin-positive, and only one cell line showed no expression of nestin; this finding corresponds with the rare occurrence of nestin-positive cells in the respective tumor sample. Moreover, three of these osteosarcoma cell lines were undoubtedly proven to be Nes+/CD133+. Our results represent the first evidence of nestin expression in osteosarcomas and suggest the possible occurrence of cells with a stem-like phenotype in these tumors.
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2.9
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