Direct quantitative analysis of nicotine alkaloids from biofluid samples using paper spray mass spectrometry.
Direct quantitative analysis of nicotine alkaloids from biofluid samples using paper spray mass spectrometry.
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DOI：
10.1021/ac402798m
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发表时间：
2013-12-03
影响因子：
7.4
通讯作者：
Ouyang, Zheng
中科院分区：
文献类型：
--
作者：
Wang, He;Ren, Yue;McLuckey, Morgan N.;Manicke, Nicholas E.;Park, Jonghyuck;Zheng, Lingxing;Shi, Riyi;Cooks, R. Graham;Ouyang, Zheng
生物体液样品中烟草衍生的尼古丁生物碱的测定对于烟草使用测试、戒烟治疗和二手烟暴露研究具有重要意义。纸喷雾质谱法（MS）已适用于直接，定量分析烟草生物碱的生物流体样品，如血液，尿液和唾液中的液体和干燥形式。尼古丁、可替宁、反式-3 ′-羟基可替宁和假木贼碱的定量限低至数ng/mL。使用高密度的打印纸基质，还实现了对新鲜血液样品的直接分析，并提高了灵敏度。定量测定大鼠血液中的可替宁进行了直接分析，使用纸喷雾和传统的分析协议，使用液相色谱MS。获得了可比的结果，这两种方法的精密度是相似的。纸喷雾MS方法是快速的，并显示出显着提高分析效率的潜力，在临床实验室，以及点的护理烟草使用评估。
The determination of tobacco derived nicotine alkaloids in biofluid samples is of great importance to testing for tobacco use, tobacco cessation treatment, and studies on exposure to secondhand smoke. Paper spray mass spectrometry (MS) has been adapted for direct, quantitative analysis of tobacco alkaloids from biofluid samples such as blood, urine and saliva in liquid and dried form. Limits of quantitation as low as several ng/mL were obtained for nicotine, cotinine, trans-3′-hydroxycotinine and anabasine. Direct analysis of fresh blood samples has also been achieved with improved sensitivity using print paper substrates of high density. Quantitation of the cotinine in the blood of a rat was performed with both direct analysis using paper spray and a traditional analysis protocol using liquid chromatography MS. Comparable results were obtained and the precision of the two methods was similar. The paper spray MS method is rapid and shows potential for significantly improved analytical efficiency in clinical labs as well as for point-of-care tobacco use assessment.
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影响因子：
7.4
作者：
Cody, RB;Laramée, JA;Durst, HD
通讯作者：
Durst, HD
影响因子：
6.7
作者：
Dempsey, D;Tutka, P;Benowitz, NL
通讯作者：
Benowitz, NL
DOI：
10.1056/nejmra0809890
发表时间：
2010-06-17
期刊：
The New England journal of medicine
影响因子：
--
作者：
Benowitz NL
通讯作者：
Benowitz NL
影响因子：
5.9
作者：
FIORE, MC
通讯作者：
FIORE, MC
影响因子：
4.2
作者：
Ifa, D. R.;Gumaelius, L. M.;Cooks, R. G.
通讯作者：
Cooks, R. G.