Interaction of quorum signals with outer membrane lipids: insights into prokaryotic membrane vesicle formation.
Interaction of quorum signals with outer membrane lipids: insights into prokaryotic membrane vesicle formation.
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DOI：
10.1111/j.1365-2958.2008.06302.x
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发表时间：
2008-07
影响因子：
3.6
通讯作者：
Whiteley M
中科院分区：
文献类型：
--
作者：
Mashburn-Warren L;Howe J;Garidel P;Richter W;Steiniger F;Roessle M;Brandenburg K;Whiteley M
Bacteria have evolved elaborate communication strategies to co-ordinate their group activities, a process termed quorum sensing (QS). Pseudomonas aeruginosa is an opportunistic pathogen that utilizes QS for diverse activities, including disease pathogenesis. P. aeruginosa has evolved a novel communication system in which the signal molecule 2-heptyl-3-hydroxy-4-quinolone (Pseudomonas Quinolone Signal, PQS) is trafficked between cells via membrane vesicles (MVs). Not only is PQS packaged into MVs, it is required for MV formation. Although MVs are involved in important biological processes aside from signalling, the molecular mechanism of MV formation is unknown. To provide insight into the molecular mechanism of MV formation, we examined the interaction of PQS with bacterial lipids. Here, we show that PQS interacts strongly with the acyl chains and 4′-phosphate of bacterial lipopolysaccharide (LPS). Using PQS derivatives, we demonstrate that the alkyl side-chain and third position hydroxyl of PQS are critical for these interactions. Finally, we show that PQS stimulated purified LPS to form liposome-like structures. These studies provide molecular insight into P. aeruginosa MV formation and demonstrate that quorum signals serve important non-signalling functions.
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影响因子：
--
作者：
Diggle, Stephen P.;Matthijs, Sandra;Williams, Paul
通讯作者：
Williams, Paul
影响因子：
56.9
作者：
Ernst, RK;Yi, EC;Miller, SI
通讯作者：
Miller, SI
DOI：
10.1179/096805102125000263
发表时间：
2002-01-01
期刊：
JOURNAL OF ENDOTOXIN RESEARCH
影响因子：
--
作者：
Jürgens, G;Müller, M;Brandenburg, K
通讯作者：
Brandenburg, K
DOI：
10.1002/masy.19880150106
发表时间：
1988-03-01
期刊：
MAKROMOLEKULARE CHEMIE-MACROMOLECULAR SYMPOSIA
影响因子：
--
作者：
KOCH, MHJ
通讯作者：
KOCH, MHJ
影响因子：
3.2
作者：
Li, ZS;Clarke, AJ;Beveridge, TJ
通讯作者：
Beveridge, TJ