An increased diversity of HCV isolates were characterized among 393 patients with liver disease in China representing six genotypes, 12 subtypes, and two novel genotype 6 variants.
An increased diversity of HCV isolates were characterized among 393 patients with liver disease in China representing six genotypes, 12 subtypes, and two novel genotype 6 variants.
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在中国393例肝病患者中，HCV分离株的多样性增加，代表六种基因型，12个亚型和两种新型基因型6变异。
DOI：
10.1016/j.jcv.2013.04.013
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发表时间：
2013-08
影响因子：
8.8
通讯作者：
Lu, Ling
中科院分区：
文献类型：
--
作者：
Gu, Lin;Tong, Wangxia;Yuan, Manqiong;Lu, Teng;Li, Chunhua;Lu, Ling
我们最近在中国南方的志愿者献血者和注射吸毒者中确定了 HCV 分离株，并揭示了基因型分布模式不仅在两个研究队列之间不同，而且与我们 2002 年采样的样本也不同。肝病患者中也可能发生模式的变化。对 393 名肝病患者的 HCV E1 和 NS5B 序列进行了表征，然后进行系统发育分析。 6种HCV基因型，12种亚型（1b：65.9％，6a：17.1％，2a：7.4％，3a：3.6％，3b：3.3％，6e：0.76％，1a，1c，2b，2f，4d和5a各0.25％），以及两种新型基因型 6 变异体被分类，显示出迄今为止发现的最复杂的 HCV 中国。尽管 1b 紧随其后的 6a 的优势与我们 2002 年采样的情况基本一致，但 1c、2f、4d、5a 的单一分离株和两种新的 HCV-6 变种的鉴定是首次报道的。除4天来自欧洲访客外，其余均来自中国患者。由于6a比例（17.1%，67/393）出人意料地低于我们最近在献血者（34.8%，82/236）和注射吸毒者（51.5%，70/136）中检测到的比例，因此进行了进一步的统计分析。平均年龄比较显示，在393名患者中，感染1b的患者显着（6.7岁）比感染6a的患者年龄显着（6.7岁），而393名患者整体年龄显着大于我们最近报道的236名献血者（8.4岁）和136名注射吸毒者（12.6岁）。解释是，年轻人感染 6a 的比例较高，而肝病患者可能比志愿者献血者和注射吸毒者更早感染。在393名肝病患者中，检测到了HCV的巨大多样性，这反映了中国HCV基因型随时间不断变化的模式。
We have recently determined HCV isolates among volunteer blood donors and IDUs in southern China and revealed the genotype distribution patterns not only different between the two studied cohorts but also from what we have sampled in 2002. A changed pattern could have also occurred among patients with liver disease. Both E1 and NS5B sequences of HCV were characterized among 393 patients with liver disease followed by phylogenetic analysis. Six HCV genotypes, 12 subtypes (1b: 65.9%, 6a: 17.1%, 2a: 7.4%, 3a: 3.6%, 3b: in 3.3%, 6e: 0.76%, and 1a, 1c, 2b, 2f, 4d, and 5a each 0.25%), and two novel genotype 6 variants were classified, showing the greatest complexity of HCV hitherto found in China. Although the predominance of 1b followed by 6a is largely consistent with what we have sampled in 2002, the identification of single isolates of 1c, 2f, 4d, 5a, and two novel HCV-6 variants were first reported. Excluding 4d from a European visitor, all the others were from Chinese patients. Since the 6a proportion (17.1%, 67/393) was unexpectedly lower than what we have recently detected among blood donors (34.8%, 82/236) and IDUs (51.5%, 70/136), further statistical analyses were conducted. Comparison of the mean ages showed that among the 393 patients, those infected with 1b were significantly (6.7 years) older than those with 6a, while the 393 patients as a whole were significantly older than the 236 blood donors (8.4 years) and 136 IDUs (12.6 years) we have recently reported. Explanations are that younger individuals had higher proportions of 6a infections while patients with liver disease could have acquired their infections earlier than volunteer blood donors and IDUs. Among 393 patients with liver disease, a great diversity in HCV was detected, which reflects a constantly changing pattern of HCV genotypes in China over time.
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