Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.
Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer.
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卵母鸡的卵巢纤维膜中恶性转化的发生率，母鸡是一种自发性卵巢癌的临床前模型。
DOI：
10.1371/journal.pone.0255007
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Barua A
中科院分区：
文献类型：
--
作者：
Paris EA;Bahr JM;Bitterman P;Basu S;Abramowicz JS;Barua A
卵巢高级浆液性癌 (HGSC) 是卵巢癌 (OVCA) 的一种致命形式。在大多数情况下，它是在晚期才被发现的，因为早期症状是非特异性的。新出现的信息表明，输卵管伞是卵巢 HGSC 的起源部位。目前可用的测试无法在早期检测卵巢 HGSC。缺乏可产生自发性卵巢 HGSC 的输卵管伞的临床前模型，是开发该疾病早期检测测试的重大障碍。本研究的目的是检查母鸡的输卵管菌毛是否是 HGSC 的起源部位，以及它是否表达患者卵巢 HGSC 中表达的几种推定标记物。使用经阴道超声 (TVUS) 成像从鸡群中选出总共 135 只蛋鸡（4 岁），然后进行安乐死并进行肉眼检查是否存在固体肿块和腹水。通过苏木精和伊红染色确定癌的组织学类型。使用免疫组织化学、免疫印迹和基因表达测定法检查正常或恶性菌毛或卵巢中 WT-1、突变体 p53、CA-125、PAX2 和 Ki67 的表达。这项研究检测到母鸡输卵管伞毛中的肿瘤，常规染色揭示了这些肿瘤中类似卵巢 HGSC 的微观特征。这些肿瘤在表达多种标志物方面与患者的卵巢 HGSC 相似。与正常菌毛相比，WT-1、突变型p53、CA-125和Ki67在菌毛肿瘤中的表达强度显着升高（P<0.05）。相反，随着肿瘤进展到晚期，PAX2的表达逐渐下降。这些标志物的表达模式与卵巢 HGSC 患者相似。因此，母鸡输卵管伞部肿瘤可能提供临床前模型来研究女性自发性卵巢 HGSC 的不同方面，包括其早期检测。
Ovarian high grade serous carcinoma (HGSC) is a lethal form of ovarian cancer (OVCA). In most cases it is detected at late stages as the symptoms are non-specific during early stages. Emerging information suggests that the oviductal fimbria is a site of origin of ovarian HGSC. Currently available tests cannot detect ovarian HGSC at early stage. The lack of a preclinical model with oviductal fimbria that develops spontaneous ovarian HGSC is a significant barrier to developing an early detection test for this disease. The goal of this study was to examine if the oviductal fimbria in hens is a site of origin of HGSC and whether it expresses several putative markers expressed in ovarian HGSC in patients. A total of 135 laying hens (4 years old) were selected from a flock using transvaginal ultrasound (TVUS) imaging, followed by euthanasia and gross examination for the presence of solid masses and ascites. Histological types of carcinomas were determined by hematoxylin and eosin staining. Expression of WT-1, mutant p53, CA-125, PAX2 and Ki67 in normal or malignant fimbriae or ovaries were examined using immunohistochemistry, immunoblotting and gene expression assays. This study detected tumors in oviductal fimbriae in hens and routine staining revealed ovarian HGSC-like microscopic features in these tumors. These tumors showed similarities to ovarian HGSC in patients in expressing several markers. Compared with normal fimbriae, intensities of expression of WT-1, mutant p53, CA-125, and Ki67 were significantly (P<0.05) higher in fimbrial tumors. In contrast, expression of PAX2 decreased gradually as the tumor progressed to late stages. The patterns of expression of these markers were similar to those in ovarian HGSC patients. Thus, tumors of the oviductal fimbria in hens may offer a preclinical model to study different aspects of spontaneous ovarian HGSC in women including its early detection.
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影响因子：
3.9
作者：
Hartkamp, Joerg;Roberts, Stefan G. E.
通讯作者：
Roberts, Stefan G. E.
影响因子：
3.3
作者：
Hakim, Amy A.;Barry, Catherine P.;Rodriguez, Gustavo C.
通讯作者：
Rodriguez, Gustavo C.
影响因子：
2.3
作者：
Barua, Animesh;Abramowicz, Jacques S.;Luborsky, Judith L.
通讯作者：
Luborsky, Judith L.
DOI：
10.7863/jum.2010.29.2.173
发表时间：
2010-02
期刊：
Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine
影响因子：
--
作者：
Barua A;Bitterman P;Bahr JM;Bradaric MJ;Hales DB;Luborsky JL;Abramowicz JS
通讯作者：
Abramowicz JS
DOI：
10.1002/prca.201300135
发表时间：
2014-10
期刊：
Proteomics. Clinical applications
影响因子：
--
作者：
Hawkridge AM
通讯作者：
Hawkridge AM