Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis.
Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis.
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血清氨基酸谱以及补充 L-精氨酸或 L-谷氨酰胺对右旋糖酐硫酸钠结肠炎的有益作用
DOI：
10.1371/journal.pone.0088335
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wu G
中科院分区：
文献类型：
--
作者：
Ren W;Yin J;Wu M;Liu G;Yang G;Xion Y;Su D;Wu L;Li T;Chen S;Duan J;Yin Y;Wu G
本研究旨在探讨葡聚糖硫酸钠（DSS）诱导的结肠炎大鼠血清氨基酸谱，以及补充不同剂量精氨酸或谷氨酰胺对结肠炎的影响。使用DSS诱导的结肠炎模型，其类似于人溃疡性结肠炎，我们在第3、7、10和12天（DSS治疗后5天）测定氨基酸的血清谱。同时，不同剂量精氨酸对大鼠的（0.4%、0.8%和1.5%）或谷氨酰胺（0.5%、1.0%和2.0%）补充对临床参数、血清氨基酸、结肠紧密连接蛋白、结肠抗氧化指标[过氧化氢酶、总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）]的影响，在第7天和第12天全面分析DSS诱导的结肠炎中的结肠促炎细胞因子[白细胞介素-1 β（IL-1β）、IL-6、IL-17和肿瘤坏死因子α（TNF-α）]。此外，使用免疫印迹分析信号转导途径的激活，包括核因子κ B（NF-κB）、促分裂原活化蛋白激酶（MAPK）、磷酸肌醇-3-激酶（PI 3 K）/PI 3 K-蛋白激酶B（Akt）和肌球蛋白轻链激酶（MLCK）-肌球蛋白轻链（MLC 20）。血清氨基酸分析显示，DSS处理改变了血清中Trp、Glu和Gln等氨基酸含量（P<0.05）。补充精氨酸或谷氨酰胺对结肠炎模型的临床和生化指标（T-SOD、IL-17和TNF-α）均有显著影响（P<0.05）。这些结果与结肠NF-κB、PI 3 K-Akt和MLCK信号通路有关。总之，精氨酸或谷氨酰胺可能是一种潜在的治疗肠道炎症性疾病。
This study was conducted to investigate serum amino acids profile in dextran sulfate sodium (DSS)-induced colitis, and impacts of graded dose of arginine or glutamine supplementation on the colitis. Using DSS-induced colitis model, which is similar to human ulcerative colitis, we determined serum profile of amino acids at day 3, 7, 10 and 12 (5 days post DSS treatment). Meanwhile, effects of graded dose of arginine (0.4%, 0.8%, and 1.5%) or glutamine (0.5%, 1.0% and 2.0%) supplementation on clinical parameters, serum amino acids, colonic tight junction proteins, colonic anti-oxidative indicators [catalase, total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px)], colonic pro-inflammatory cytokines [interleukin-1 beta (IL-1β), IL-6, IL-17 and tumor necrosis factor alpha (TNF-α)] in DSS-induced colitis were fully analyzed at day 7 and 12. Additionally, the activation of signal transduction pathways, including nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK), phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt), and myosin light chain kinase (MLCK)- myosin light chain (MLC20), were analyzed using immunoblotting. Serum amino acids analysis showed that DSS treatment changed the serum contents of amino acids, such as Trp, Glu, and Gln (P<0.05). Dietary arginine or glutamine supplementation had significant (P<0.05) influence on the clinical and biochemical parameters (T-SOD, IL-17 and TNF-α) in colitis model. These results were associated with colonic NF-κB, PI3K-Akt and MLCK signaling pathways. In conclusion, arginine or glutamine could be a potential therapy for intestinal inflammatory diseases.
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