Immunohistochemical Characteristics of the Human Carotid Body in the Antenatal and Postnatal Periods of Development.
Immunohistochemical Characteristics of the Human Carotid Body in the Antenatal and Postnatal Periods of Development.
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DOI：
10.3390/ijms22158222
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发表时间：
2021-07-30
影响因子：
5.6
通讯作者：
Saveliev S
中科院分区：
文献类型：
--
作者：
Otlyga D;Tsvetkova E;Junemann O;Saveliev S
颈动脉体的进化和个体发育仍然是研究不足。旨在研究不同动物物种个体发育不同时期器官的比较形态学的研究应该在理解颈动脉体的生理学方面发挥关键作用。然而，尽管有两个多世纪的研究，人类颈动脉体仍然知之甚少。在这一结构的产前发育方面尤其存在许多知识空白。本研究的目的是研究人颈动脉体在出生前后发育过程中的形态学和免疫组织化学特征。我们研究了1个胚胎，20个胎儿和13个不同年龄的成人的颈动脉体标本在尸检中获得。免疫组化结果显示，β Ⅲ-微管蛋白和酪氨酸羟化酶在各发育时期的Ⅰ型细胞和神经纤维中均有表达，突触素和PGP9.5在部分产前病例和全部产后病例的Ⅰ型细胞中均有表达，200 kDa神经丝在部分产前病例和全部产后病例的神经纤维中均有表达，而200 kDa神经丝在部分产前病例和全部产后病例的Ⅰ型细胞中均有表达。Ⅱ型细胞和雪旺细胞中GFAP和S100在部分产前病例和全部产后病例中均有表达。高水平的酪氨酸羟化酶的I型细胞是一个显着的特点，产前颈动脉体。相反，在成人的I型细胞中，酪氨酸羟化酶的表达显著降低。我们的数据表明，人类颈动脉体可能在产前期间执行内分泌功能，而在出生后的发育期，它失去了这一功能，成为化学感受器官。
The evolutionary and ontogenetic development of the carotid body is still understudied. Research aimed at studying the comparative morphology of the organ at different periods in the individual development of various animal species should play a crucial role in understanding the physiology of the carotid body. However, despite more than two centuries of study, the human carotid body remains poorly understood. There are many knowledge gaps in particular related to the antenatal development of this structure. The aim of our work is to study the morphological and immunohistochemical characteristics of the human carotid body in the antenatal and postnatal periods of development. We investigated the human carotid bodies from 1 embryo, 20 fetuses and 13 adults of different ages using samples obtained at autopsy. Immunohistochemistry revealed expression of βIII-tubulin and tyrosine hydroxylase in the type I cells and nerve fibers at all periods of ontogenesis; synaptophysin and PGP9.5 in the type I cells in some of the antenatal cases and all of the postnatal cases; 200 kDa neurofilaments in nerve fibers in some of the antenatal cases and all of the postnatal cases; and GFAP and S100 in the type II cells and Schwann cells in some of the antenatal cases and all of the postnatal cases. A high level of tyrosine hydroxylase in the type I cells was a distinctive feature of the antenatal carotid bodies. On the contrary, in the type I cells of adults, the expression of tyrosine hydroxylase was significantly lower. Our data suggest that the human carotid body may perform an endocrine function in the antenatal period, while in the postnatal period of development, it loses this function and becomes a chemosensory organ.
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影响因子：
7.7
作者：
Hockman, Dorik;Burns, Alan J.;Baker, Clare V. H.
通讯作者：
Baker, Clare V. H.
影响因子：
2.9
作者：
MONTIBLOCH, L;STENSAAS, LJ;EYZAGUIRRE, C
通讯作者：
EYZAGUIRRE, C
影响因子：
5.5
作者：
BLANCO, CE;DAWES, GS;MCCOOKE, HB
通讯作者：
MCCOOKE, HB
影响因子：
2.5
作者：
MCDONALD, DM;MITCHELL, RA
通讯作者：
MITCHELL, RA
DOI：
10.1111/nyas.12780
发表时间：
2016-01-01
期刊：
SPECIAL ISSUE: RESPIRATORY SCIENCE
影响因子：
--
作者：
Jonz, Michael G.;Buck, Leslie T.;Zaccone, Giacomo
通讯作者：
Zaccone, Giacomo