Potent Osteogenesis and Chondrogenesis of CD34-Enriched Mouse Adipose-Derived Stem Cells
Potent Osteogenesis and Chondrogenesis of CD34-Enriched Mouse Adipose-Derived Stem Cells
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富含 CD34 的小鼠脂肪干细胞的有效成骨和软骨形成作用
DOI：
10.5405/jmbe.1581
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Xiong Y;Liu W
脂肪源性干细胞（ASCs），获得使用广泛报道的培养方法，实际上是一个杂合细胞群的脂肪基质血管部分（SVF）。这种异质性可能会干扰脂肪间充质干细胞（ADMSCs）的多向分化潜能。因此，有必要建立一种有效的方法来分离具有高多向分化潜能的ADMSC。多项研究表明，粘液唾液酸蛋白（mucosialin，CD 34）可能是ADMSCs的特异性标志物之一。在我们以前的研究中，在毛囊形态发生模型中，从合并的SVF中分选出的富含CD 34的细胞群比未分选的细胞具有更强的分化潜力。然而，目前尚不清楚富含CD 34的细胞是否具有更强的其他谱系分化的潜力，特别是成骨和软骨形成。在这项研究中，从小鼠脂肪来源的细胞中收集并分选出CD 34 ^+细胞，并使用工程化骨和软骨模型检测其成骨和软骨形成的潜力。结果表明，当接种在s-磷酸三钙上时，CD 34 ^+细胞表现出更强的成骨潜力，货车Gieson和胶原I染色比CD 34 ^-细胞和未分选的SVF更强。此外，CD 34 ^+细胞也表现出比CD 34 ^-细胞和未分选的SVF更强的软骨形成潜力，在软骨细胞团和工程化软骨中甲苯胺蓝、番红-O和胶原II的染色更强。最重要的是，只有CD 34 ^+细胞可以形成软骨样陷窝结构。所有这些结果表明，CD 34可以作为一个特定的表面标记，富集ADMSC在ASCs。
Adipose-derived stem cells (ASCs) obtained using a widely reported culture method are actually a heterozygous cell population of the adipose stromal vascular fraction (SVF). This heterogeneity may interfere with the multi-differentiation potential of adipose-derived mesenchymal stem cells (ADMSCs). It is therefore necessary to establish an efficient method for isolating ADMSCs that have high multi-differentiation potential. Several studies indicated that mucosialin (CD34) might be one of the specific markers of ADMSCs. In our previous studies, a CD34-enriched cell population sorted from the pooled SVF had stronger differentiation potential than that of unsorted cells in a hair follicle morphogenesis model. However, it remains unclear whether CD34-enriched cells have stronger potential for other lineage differentiation, particularly osteogenesis and chondrogenesis. In this study, CD34^+ cells were harvested and sorted from murine adipose-derived cells and their potentials for osteogenesis and chondrogesis were examined using engineered bone and cartilage models. The results show that CD34^+ cells exhibited stronger potential for bone formation with stronger van Gieson and collagen I staining than those of CD34^- cells and an unsorted SVF when seeded on s-tricalcium phosphate. Furthermore, CD34^+ cells also exhibited a much stronger chondrogenic potential than those of CD34^- cells and an unsorted SVF, with stronger staining of toluidine blue, Safranin-O, and collagen II in a chondrocyte pellet and engineered cartilage. Most importantly, only CD34^+ cells could form a cartilage-like lacunae structure. All these results indicate that CD34 may serve as a specific surface marker for enriching ADMSCs in ASCs.
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