Tanycyte-like cells form a blood-cerebrospinal fluid barrier in the circumventricular organs of the mouse brain.
Tanycyte-like cells form a blood-cerebrospinal fluid barrier in the circumventricular organs of the mouse brain.
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DOI：
10.1002/cne.23355
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发表时间：
2013-10-15
影响因子：
2.5
通讯作者：
Dehouck, Benedicte
中科院分区：
文献类型：
--
作者：
Langlet, Fanny;Mullier, Amandine;Bouret, Sebastien G.;Prevot, Vincent;Dehouck, Benedicte
关键词：
伸展细胞是高度特化的室管膜细胞，其在位于下丘脑结节区的室周器官（CVO）正中隆起（ME）水平上形成血-脑脊液（CSF）屏障。该室管膜层具有组织良好的紧密连接，这是中枢神经系统屏障的标志，其在ME的有孔门静脉中缺乏。屏障特性从血管到心室侧的位移允许血液传播的分子扩散到ME的实质中，而伸展细胞紧密连接控制其扩散到CSF中，从而维持脑内稳态。在目前的工作中，我们结合免疫组织化学和通透性研究，探讨存在的伸展细胞屏障沿着其他脑CVOs的心室壁。我们的数据表明，不像立方室管膜细胞，室管膜细胞接壤的CVOs具有长的过程，项目到实质的CVOs，达到有孔毛细血管网络。值得注意的是，这些伸长体样细胞在其细胞体周围显示出组织良好的紧密连接。与这些观察结果一致，渗透性研究表明，该室管膜层作为扩散屏障。总之，我们的研究结果表明，伸长细胞是所有CVOs的一个特征，并产生潜在的新的见解，他们参与调节血液，大脑和CSF之间的交换，在这些“脑窗”。
Tanycytes are highly specialized ependymal cells that form a blood–cerebrospinal fluid (CSF) barrier at the level of the median eminence (ME), a circumventricular organ (CVO) located in the tuberal region of the hypothalamus. This ependymal layer harbors well-organized tight junctions, a hallmark of central nervous system barriers that is lacking in the fenestrated portal vessels of the ME. The displacement of barrier properties from the vascular to the ventricular side allows the diffusion of blood-borne molecules into the parenchyma of the ME while tanycyte tight junctions control their diffusion into the CSF, thus maintaining brain homeostasis. In the present work, we combined immunohistochemical and permeability studies to investigate the presence of tanycyte barriers along the ventricular walls of other brain CVOs. Our data indicate that, unlike cuboidal ependymal cells, ependymal cells bordering the CVOs possess long processes that project into the parenchyma of the CVOs to reach the fenestrated capillary network. Remarkably, these tanycyte-like cells display well-organized tight junctions around their cell bodies. Consistent with these observations, permeability studies show that this ependymal layer acts as a diffusion barrier. Together, our results suggest that tanycytes are a characteristic feature of all CVOs and yield potential new insights into their involvement in regulating the exchange between the blood, the brain, and the CSF within these “brain windows.”
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影响因子：
7.8
作者：
Furuse, Mikio;Hata, Masaki;Furuse, Kyoko;Yoshida, Yoko;Haratake, Akinori;Sugitani, Yoshinobu;Noda, Tetsuo;Kubo, Akiharu;Tsukita, Shoichiro
通讯作者：
Tsukita, Shoichiro
影响因子：
5.3
作者：
De Seranno, S;Estrella, C;Prevot, V
通讯作者：
Prevot, V
DOI：
10.1111/j.1749-6632.1988.tb51417.x
发表时间：
1988-01-01
影响因子：
5.2
作者：
Goldstein, G W
通讯作者：
Goldstein, G W
影响因子：
2.5
作者：
Ciofi, Philippe
通讯作者：
Ciofi, Philippe
影响因子：
2.5
作者：
Caron, Emilie;Sachot, Christelle;Bouret, Sebastien G.
通讯作者：
Bouret, Sebastien G.