A base substitution in OsphyC disturbs its Interaction with OsphyB and affects flowering time and chlorophyll synthesis in rice.
A base substitution in OsphyC disturbs its Interaction with OsphyB and affects flowering time and chlorophyll synthesis in rice.
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在Isphyc中的基本取代会扰乱其与Isphyb的相互作用，并影响大米中的开花时间和叶绿素合成。
DOI：
10.1186/s12870-022-04011-y
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发表时间：
2022-12-27
影响因子：
5.3
通讯作者：
Xu, Jie
中科院分区：
文献类型：
--
作者：
Lin, Xiaoli;Huang, Yongping;Rao, Yuchun;Ouyang, Linjuan;Zhou, Dahu;Zhu, Changlan;Fu, Junru;Chen, Chunlian;Yin, Jianhua;Bian, Jianmin;He, Haohua;Zou, Guoxing;Xu, Jie
光敏色素是植物重要的光感受器，在植物光形态建成中起着重要作用。PhyA和PhyB在植物中的功能已被充分研究，而PhyC在植物中的功能还不清楚。对水稻晚抽穗3号突变体进行了鉴定，并利用图位克隆技术对LHD 3基因进行了克隆。LHD 3编码水稻光敏色素C。氨基酸取代OSphyC破坏其与OSphyB或本身的相互作用，抑制同二聚体或异二聚体形成的功能形式。与野生型植株相比，lhd 3突变体在长日照和短日照条件下均表现出延迟开花的特性，且延迟开花时间通过Ehd 1途径与日照长度呈正相关。此外，lhd 3在变绿过程中表现出淡绿色的叶片表型和较慢的叶绿素合成速率。许多与光周期介导的开花和叶绿素合成有关的关键基因的转录模式在lhd 3中发生了改变。OsPhyC的二聚化对于其在叶绿素合成和抽穗调节中的功能是重要的。我们的研究结果将有助于进一步阐明OSphyC在水稻光信号转导中的作用和机制。在线版本包含补充材料，可通过10.1186/s12870-022-04011-y获得。
Phytochromes are important photoreceptors in plants, and play essential roles in photomorphogenesis. The functions of PhyA and PhyB in plants have been fully analyzed, while those of PhyC in plant are not well understood. A rice mutant, late heading date 3 (lhd3), was characterized, and the gene LHD3 was identified with a map-based cloning strategy. LHD3 encodes phytochrome C in rice. Animo acid substitution in OsphyC disrupted its interaction with OsphyB or itself, restraining functional forms of homodimer or heterodimer formation. Compared with wild-type plants, the lhd3 mutant exhibited delayed flowering under both LD (long-day) and SD (short-day) conditions, and delayed flowering time was positively associated with the day length via the Ehd1 pathway. In addition, lhd3 showed a pale-green-leaf phenotype and a slower chlorophyll synthesis rate during the greening process. The transcription patterns of many key genes involved in photoperiod-mediated flowering and chlorophyll synthesis were altered in lhd3. The dimerization of OsPhyC is important for its functions in the regulation of chlorophyll synthesis and heading. Our findings will facilitate efforts to further elucidate the function and mechanism of OsphyC and during light signal transduction in rice. The online version contains supplementary material available at 10.1186/s12870-022-04011-y.
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影响因子：
11.6
作者：
Takano, M;Inagaki, N;Shinomura, T
通讯作者：
Shinomura, T
影响因子：
16.6
作者：
Qiu Y;Pasoreck EK;Reddy AK;Nagatani A;Ma W;Chory J;Chen M
通讯作者：
Chen M
影响因子：
11.6
作者：
EDGERTON, MD;JONES, AM
通讯作者：
JONES, AM
DOI：
10.1073/pnas.0404286101
发表时间：
2004-08-03
影响因子：
11.1
作者：
Sharrock, RA;Clack, T
通讯作者：
Clack, T
影响因子：
64.8
作者：
Matsushita, T;Mochizuki, N;Nagatani, A
通讯作者：
Nagatani, A