Effects of adipokinetic hormone and its related peptide on maintaining hemolymph carbohydrate and lipid levels in the two-spotted cricket, Gryllus bimaculatus

Effects of adipokinetic hormone and its related peptide on maintaining hemolymph carbohydrate and lipid levels in the two-spotted cricket, Gryllus bimaculatus
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脂肪运动激素及其相关肽对维持双斑蟋蟀血淋巴碳水化合物和脂质水平的影响

DOI:
10.1080/09168451.2017.1422106
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发表时间:
2018
期刊:
Bioscience, Biotechnology, and Biochemistry
影响因子:
--
通讯作者:
Nagata Shinji
Nagata Shinji
中科院分区:
--
文献类型:
--
作者:
Zhou Yi Jun;Fukumura Keisuke;Nagata Shinji

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脂肪代谢激素(Adipokinetic hormone,AKH)通过从脂肪体中动员脂肪和碳水化合物来调节昆虫体内的能量平衡。在这里,使用RNA测序数据,我们确定了编码AKH(GbAKH)和它的高度同源的AKH/corazonin相关肽(GbACP)的cDNAs在身体的心脏的两点蟋蟀,蟋蟀bimaculatus。RT-PCR结果显示GbAK和GbACP主要表达于心侧体和咽侧体。系统发育分析表明,GbAKH和GbACP分别属于含有其他AKH和ACP的进化枝。注射合成GbAKH和GbACP可显著升高血淋巴碳水化合物和脂质水平,并减少食物摄入。相反,通过RNA干扰敲低GbAK和GbACP增加了食物摄入,尽管血淋巴脂质水平没有改变。总的来说,这项研究提供的证据表明,ACP调节血淋巴中的碳水化合物和脂质水平的板球,可能与AKH的能量稳态的维持合作的贡献。
Adipokinetic hormone (AKH) regulates energy homeostasis in insects by mobilizing lipid and carbohydrate from the fat body. Here, using RNA sequencing data, we identified cDNAs encodingAKH(GbAKH) and its highly homologous hormoneAKH/corazonin-related peptide(GbACP) in the corpora cardiaca of the two-spotted cricket,Gryllus bimaculatus. RT-PCR revealed thatGbAKHandGbACPare predominantly expressed in the corpora cardiaca and corpora allata, respectively. Phylogenetic analysis confirmed that the identified GbAKH and GbACP belong to the clades containing other AKHs and ACPs, respectively. Injection of synthetic GbAKH and GbACP elevated hemolymph carbohydrate and lipid levels and reduced food intake significantly. In contrast, knockdown ofGbAKHandGbACPby RNA interference increased the food intake, although hemolymph lipid level was not altered. Collectively, this study provides evidence that ACP regulates hemolymph carbohydrate and lipid levels in cricket, possibly collaborative contribution with AKH to the maintenance of energy homeostasis.
不会飞的翅多形虫 Pyrrocoris apterus (L.) 中枢神经系统和血淋巴中脂肪运动激素的发育和昼夜变化。
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