HIV-1 diversity, drug-resistant mutations, and viral evolution among high-risk individuals in phase II HIV vaccine trial sites in southern China.
HIV-1 diversity, drug-resistant mutations, and viral evolution among high-risk individuals in phase II HIV vaccine trial sites in southern China.
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DOI：
10.1371/journal.pone.0068656
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yu XF
中科院分区：
文献类型：
--
作者：
Qi H;Zhao K;Xu F;Zhang X;Zhang Z;Yang L;Li C;Liang X;Guo W;Chen S;Liu Z;Zhang W;Yu XF
自1996年报告首例病例以来，中国广西的HIV-1流行率一直在上升。广西超过一半的HIV-1阳性患者是注射吸毒者（IDUs），可能是因为该省靠近贩毒路线。由于广西正在进行二期艾滋病毒疫苗试验，目前对注射吸毒者中艾滋病毒-1亚型的描述将为未来的艾滋病毒疫苗试验以及进一步控制和预防艾滋病毒-1传播提供关键信息。为此，我们对广西多个城市2008-2010年注射吸毒者中HIV-1样本进行了分子流行病学调查。基于gag/pol片段的研究结果表明，IDUs中HIV-1 CRF08_BC的比例非常高（78.47%），CRF01_AE的比例非常低（15.38%），CRF07_BC的比例很低（6.15%）。在最近的idu中，CRF08 HIV-1毒株的高比例与候选疫苗构建相匹配。然而，未来的疫苗开发还应纳入crf01靶向候选疫苗。CRF08_BC和CRF01_AE的Env序列进化模式不同，表明这两种HIV-1亚型受到不同的局部选择压力。独特的耐药突变也被检测到，我们的数据表明，HIV治疗方案应该考虑预先存在的耐药突变。
HIV-1 prevalence in Guangxi, China, has been growing since 1996, when the first case was reported. Over half of HIV-1 positive patients in Guangxi Province were injecting drug users (IDUs), possibly because of the province’s location near drug-trafficking routes. Since a phase II HIV vaccine trial is ongoing there, a current characterization of the subtypes of HIV-1 among IDUs in Guangxi would provide critical information for future HIV vaccine trials, as well as further control and prevention of HIV-1 transmission. Thus, we conducted a molecular epidemiological investigation of HIV-1 samples from 2008–2010 among IDUs in multiple cities in Guangxi Province. Our results, based on the gag/pol fragment, indicated a very high proportion (78.47%) of HIV-1 CRF08_BC recombinants, some CRF01_AE (15.38%) recombinants, and a low proportion of CRF07_BC (6.15%) recombinants among the IDUs. The high proportion of CRF08 HIV-1 strains among recent IDUs matches the vaccine candidate constructs. However, future vaccine development should also incorporate CRF01-targeted vaccine candidates. Distinct Env sequence evolution patterns were observed for CRF08_BC and CRF01_AE, indicating that different local selection pressures have been exerted on these two HIV-1 subtypes. Unique drug-resistant mutations were also detected, and our data indicate that HIV treatment programs should consider pre-existing drug-resistant mutations.
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影响因子：
3.7
作者：
Shang H;Zhong P;Liu J;Han X;Dai D;Zhang M;Zhao K;Xu R;Yu XF
通讯作者：
Yu XF
影响因子：
11.8
作者：
Garten, RJ;Zhang, JB;Yu, XF
通讯作者：
Yu, XF
影响因子：
3.8
作者：
Pang, Wei;Zhang, Chiyu;Zheng, Yong-Tang
通讯作者：
Zheng, Yong-Tang
影响因子：
--
作者：
Teng, T;Shao, Y
通讯作者：
Shao, Y
谱系地理学分析揭示新疆在亚洲 HIV-1 CRF07_BC 和 HCV 3a 传播中的关键作用
影响因子：
3.7
作者：
Liu J;Zhang C
通讯作者：
Zhang C