Dynamic Distribution of Gut Microbiota in Goats at Different Ages and Health States.
Dynamic Distribution of Gut Microbiota in Goats at Different Ages and Health States.
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DOI：
10.3389/fmicb.2018.02509
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发表时间：
2018
影响因子：
5.2
通讯作者：
Zhu R
中科院分区：
文献类型：
--
作者：
Wang Y;Zhang H;Zhu L;Xu Y;Liu N;Sun X;Hu L;Huang H;Wei K;Zhu R
动物肠道微生物群 (GM) 的重要性因其在新陈代谢、免疫和健康维护中的关键作用而得到广泛认可。健康水平可以通过GM的动态分布来体现。在本研究中，利用细菌16S rRNA基因的高通量测序来比较健康和腹泻儿童粪便中的微生物种群，反映了儿童微生物群的动态变化，并研究了与成年健康山羊的差异。根据操作分类单元分析、α多样性和β多样性的结果，健康的孩子和山羊不仅表现出更高的物种丰富度，而且比腹泻的孩子表现出更高的细菌多样性。厚壁菌门和拟杆菌门是所有样本中最主要的门。在属水平上，逐渐观察到腹泻儿童与其他两组之间多样性和丰度的差异。腹泻仔猪肠道中，拟杆菌属仍为优势菌种，梭状芽孢杆菌_sensu_stricto_1和类芽孢杆菌所占比例增加，而理肯氏菌科_RC9_gut_group、瘤胃球菌科_UCG-005和克里斯滕氏菌科_R-7_组比例显着减少。结果表明，腹泻儿童和健康儿童的GM差异显着，而儿童和山羊的GM差异不明显。肠道微生物群组成的差异可能不是腹泻的原因，细菌丰富度的一些变化可能指导我们对腹泻的解释。本研究首次调查了不同年龄和健康状况的波尔山羊中转基因的分布情况。此外，本研究将为建立山羊腹泻防治体系提供理论依据。
The importance of the gut microbiota (GM) of animals is widely acknowledged because of its pivotal roles in metabolism, immunity, and health maintenance. The level of health can be reflected by the dynamic distribution of GM. In this study, high-throughput sequencing of the bacterial 16S rRNA gene was used to compare the microbial populations from feces in healthy and diarrheic kids, which reflected the dynamic shift of microbiota in kids and investigated differences from adult healthy goats. Healthy kids and goats not only displayed higher species richness but also exhibited higher bacterial diversity than diarrheic kids based on the results of the operational taxonomic unit analysis, alpha diversity, and beta diversity. Firmicutes and Bacteroidetes were the most dominant phyla in all samples. At the genus level, the differences in diversity and abundance between diarrheic kids and the other two groups were gradually observed. In the diarrheic kid intestine, Bacteroides remained the dominant species, and the proportion of Clostridium_sensu_stricto_1 and Paeniclostridium increased, whereas Rikenellaceae_RC9_gut_group, Ruminococcaceae_UCG-005, and Christensenellaceae_R-7_group were significantly reduced. The results showed the differences of GM in diarrheic kids and healthy kids were significant while in kids and goats were not obvious. Differences in the composition of intestinal microbiota may not be the cause of diarrhea, and some changes of bacterial richness may guide our interpretation of diarrhea. This study is the first to investigate the distribution of GM in Boer goats with different ages and health states. Furthermore, this study will provide a theoretical basis for the establishment of a prevention and treatment system for goat diarrhea.
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影响因子：
4.6
作者：
Bretin A;Lucas C;Larabi A;Dalmasso G;Billard E;Barnich N;Bonnet R;Nguyen HTT
通讯作者：
Nguyen HTT
怀孕、出生和婴儿期的微生物变化。
影响因子：
5.2
作者：
Nuriel-Ohayon M;Neuman H;Koren O
通讯作者：
Koren O
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.
影响因子：
16.6
作者：
Dubin K;Callahan MK;Ren B;Khanin R;Viale A;Ling L;No D;Gobourne A;Littmann E;Huttenhower C;Pamer EG;Wolchok JD
通讯作者：
Wolchok JD
影响因子：
11.4
作者：
Lopez-Gonzalez, J. A.;Suarez-Estrella, F.;Moreno, J.
通讯作者：
Moreno, J.