Two eisosome proteins play opposite roles in autophagic control and sustain cell integrity, function and pathogenicity in Beauveria bassiana
Two eisosome proteins play opposite roles in autophagic control and sustain cell integrity, function and pathogenicity in Beauveria bassiana
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两种胞体蛋白在白僵菌的自噬控制和维持细胞完整性、功能和致病性中发挥相反的作用
DOI：
10.1111/1462-2920.13727
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发表时间：
2017-05
影响因子：
5.1
通讯作者：
Feng Ming-Guang
中科院分区：
文献类型：
--
作者：
Zhang Long-Bin;Tang Li;Ying Sheng-Hua;Feng Ming-Guang
Pil1A and Pil1B are two core eisosome proteins that are homologous to yeast Pil1/Lsp1 or filamentous fungal Pil1A/Pil1B but have been unexplored in entomopathogenic fungi. Here we examined subcellular localization and functions of Pil1A and Pil1B in Beauveria bassiana, a fungal insect pathogen. Either localization or co-localization experiments of the two proteins demonstrated that Pil1A and Pil1B were simultaneously localized at the periphery of hyphal cells for formation of stable, punctuate spots in B. bassiana. This is different from a reliance of proper Lsp1/Pil1B localization upon Pil1/Pil1A in other fungi. Deletions of pil1A and pil1B caused opposite changes in expression of many autophagy-related genes and formation of intravacuolar autophagosomes. Such opposite changes were restored to nearly normal status by exogenous rapamycin, implicating a link of Pil1A/B to the target of rapamycin signalling pathway. All single/double deletion mutants of pil1A and pil1B lost almost all pathogenicity due to reduced ability to secrete Pr1 proteases for cuticle degradation. They also showed differential changes in cell wall integrity and multiple stress responses. These findings unveil opposite roles for Pil1A and Pil1B in autophagic regulation and an essentiality of both for cell integrity, function and pathogenicity of the fungal entomopathogen.
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白僵菌真菌昆虫致病性进化的基因组视角
影响因子：
4.6
作者：
Xiao G;Ying SH;Zheng P;Wang ZL;Zhang S;Xie XQ;Shang Y;St Leger RJ;Zhao GP;Wang C;Feng MG
通讯作者：
Feng MG
自噬相关基因BbATG1和BbATG8在真菌病原白僵菌的分化、抗逆性和毒力方面具有不同的功能
影响因子：
4.6
作者：
Ying SH;Liu J;Chu XL;Xie XQ;Feng MG
通讯作者：
Feng MG
DOI：
10.1083/jcb.201104040
发表时间：
2011-11-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Karotki L;Huiskonen JT;Stefan CJ;Ziółkowska NE;Roth R;Surma MA;Krogan NJ;Emr SD;Heuser J;Grünewald K;Walther TC
通讯作者：
Walther TC
影响因子：
3.6
作者：
Frohner IE;Bourgeois C;Yatsyk K;Majer O;Kuchler K
通讯作者：
Kuchler K
影响因子：
3.6
作者：
Puttikamonkul S;Willger SD;Grahl N;Perfect JR;Movahed N;Bothner B;Park S;Paderu P;Perlin DS;Cramer RA Jr
通讯作者：
Cramer RA Jr