Differential expression proteomics to investigate responses and resistance to Orobanche crenata in Medicago truncatula.
Differential expression proteomics to investigate responses and resistance to Orobanche crenata in Medicago truncatula.
复制标题
DOI：
10.1186/1471-2164-10-294
复制
发表时间：
2009-07-03
期刊：
影响因子：
4.4
通讯作者：
Jorrín JV
中科院分区：
文献类型：
--
作者：
Castillejo MA;Maldonado AM;Dumas-Gaudot E;Fernández-Aparicio M;Susín R;Diego R;Jorrín JV
摘要被子植物齿列当的寄生侵染是制约世界范围内豆科植物栽培的主要因素。迄今所达到的保护程度不是不完全就是短暂的。因此，一个有效的控制寄生虫需要更好地了解其相互作用和相关的耐药机制在分子水平上。为了研究植物对这种寄生植物的反应和抗性的分子基础，我们使用了蛋白质组学方法。在两个时间点（感染后21和25天），在对照以及接种的植物中，两个加入的模式豆科植物蒺藜苜蓿的根蛋白质组显示其抗性表型的差异进行了比较。我们报告定量以及定性差异的2-DE图之间的早期（SA 27774）和晚期抗性（SA 4087）基因型考马斯和银染后：69个差异点之间观察到未接种的基因型，42和25点SA 4087和SA 27774未接种和接种的植物，分别。在MALDI-TOF/TOF质谱后，通过肽质量指纹（PMF）鉴定了49个差异点。许多在基因型之间和寄生虫感染后表现出显着差异的蛋白质属于防御和应激相关蛋白的功能类别。许多斑点对应于具有相同功能的蛋白质，并且可能代表多基因家族的成员或相同蛋白质的转录后形式。获得的结果表明，存在一个通用的防御机制，在感染的早期阶段和不同的两种基因型。在SA 27774基因型中观察到的对感染的更快反应可能是由于针对寄生虫成功感染所需的关键元件的蛋白质的作用，例如蛋白酶抑制剂。我们的数据进行了讨论，并与以前获得的豌豆和其他植物病原体和植物寄生植物系统的转录组学分析。
Parasitic angiosperm Orobanche crenata infection represents a major constraint for the cultivation of legumes worldwide. The level of protection achieved to date is either incomplete or ephemeral. Hence, an efficient control of the parasite requires a better understanding of its interaction and associated resistance mechanisms at molecular levels. In order to study the plant response to this parasitic plant and the molecular basis of the resistance we have used a proteomic approach. The root proteome of two accessions of the model legume Medicago truncatula displaying differences in their resistance phenotype, in control as well as in inoculated plants, over two time points (21 and 25 days post infection), has been compared. We report quantitative as well as qualitative differences in the 2-DE maps between early- (SA 27774) and late-resistant (SA 4087) genotypes after Coomassie and silver-staining: 69 differential spots were observed between non-inoculated genotypes, and 42 and 25 spots for SA 4087 and SA 27774 non-inoculated and inoculated plants, respectively. In all, 49 differential spots were identified by peptide mass fingerprinting (PMF) following MALDI-TOF/TOF mass spectrometry. Many of the proteins showing significant differences between genotypes and after parasitic infection belong to the functional category of defense and stress-related proteins. A number of spots correspond to proteins with the same function, and might represent members of a multigenic family or post-transcriptional forms of the same protein. The results obtained suggest the existence of a generic defense mechanism operating during the early stages of infection and differing in both genotypes. The faster response to the infection observed in the SA 27774 genotype might be due to the action of proteins targeted against key elements needed for the parasite's successful infection, such as protease inhibitors. Our data are discussed and compared with those previously obtained with pea and transcriptomic analysis of other plant-pathogen and plant-parasitic plant systems.
登录
查看更多内容
影响因子：
3.8
作者：
Chevalier, F;Rofidal, V;Rossignol, M
通讯作者：
Rossignol, M
影响因子：
3.4
作者：
Dumas-Gaudot, E;Amiour, N;Gianinazzi, S
通讯作者：
Gianinazzi, S
影响因子：
3.5
作者：
Anand, A;Lei, ZT;Muthukrishnan, S
通讯作者：
Muthukrishnan, S
影响因子：
3.8
作者：
Bestel-Corre, G;Gianinazzi, S;Dumas-Gaudot, E
通讯作者：
Dumas-Gaudot, E
影响因子：
5.1
作者：
Colditz, F;Nyamsuren, O;Krajinski, F
通讯作者：
Krajinski, F