Secondary compound hypothesis revisited: Selected plant secondary metabolites promote bacterial degradation of cis-1,2-dichloroethylene (cDCE).
Secondary compound hypothesis revisited: Selected plant secondary metabolites promote bacterial degradation of cis-1,2-dichloroethylene (cDCE).
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DOI：
10.1038/s41598-017-07760-1
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发表时间：
2017-08-16
影响因子：
4.6
通讯作者：
Uhlik O
中科院分区：
文献类型：
--
作者：
Fraraccio S;Strejcek M;Dolinova I;Macek T;Uhlik O
顺式-1，2-二氯乙烯（Cis-1，2-dichloroethylene，cDCE）是一种常见的有害化合物，在高氯乙烯（CEs）的不完全还原脱氯过程中会在污染场地中积累。简单的单芳烃，如甲苯和苯酚，已被证明在没有其他碳源的情况下不能降解cDCE的微生物群落中诱导cDCE的生物转化。这项基于微观的实验室研究的目标是发现无毒的天然单芳香族次生植物代谢物（SPME），可以以类似于甲苯和苯酚的方式增强cDCE降解。根据它们的单芳香分子结构和在自然界中广泛存在的情况，选择了8种SPME。选择支持细菌生长和促进cDCE降解的SPME的适用性在从cDCE污染的土壤中富集的需氧微生物培养物中进行了评价，其中存在每种测试的SPME和cDCE。在富含苯乙酮、苯乙醇、对羟基苯甲酸和反式肉桂酸的培养物中实现了显著的cDCE消耗。每个微生物群落的16 S rRNA基因序列分析揭示了属于贪铜菌属、红球菌属、伯克霍尔德菌属、不动杆菌属和假单胞菌属的细菌的普遍富集。我们的研究结果进一步证实了先前提出的次级化合物假说，即植物代谢产物释放到根际可以引发环境污染物的生物降解，包括cDCE。
Cis-1,2-dichloroethylene (cDCE), which is a common hazardous compound, often accumulates during incomplete reductive dechlorination of higher chlorinated ethenes (CEs) at contaminated sites. Simple monoaromatics, such as toluene and phenol, have been proven to induce biotransformation of cDCE in microbial communities incapable of cDCE degradation in the absence of other carbon sources. The goal of this microcosm-based laboratory study was to discover non-toxic natural monoaromatic secondary plant metabolites (SPMEs) that could enhance cDCE degradation in a similar manner to toluene and phenol. Eight SPMEs were selected on the basis of their monoaromatic molecular structure and widespread occurrence in nature. The suitability of the SPMEs chosen to support bacterial growth and to promote cDCE degradation was evaluated in aerobic microbial cultures enriched from cDCE-contaminated soil in the presence of each SPME tested and cDCE. Significant cDCE depletions were achieved in cultures enriched on acetophenone, phenethyl alcohol, p-hydroxybenzoic acid and trans-cinnamic acid. 16S rRNA gene sequence analysis of each microbial community revealed ubiquitous enrichment of bacteria affiliated with the genera Cupriavidus, Rhodococcus, Burkholderia, Acinetobacter and Pseudomonas. Our results provide further confirmation of the previously stated secondary compound hypothesis that plant metabolites released into the rhizosphere can trigger biodegradation of environmental pollutants, including cDCE.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
4.4
作者：
Gilbert, ES;Crowley, DE
通讯作者：
Crowley, DE
DOI：
10.1007/s11157-004-4733-8
发表时间：
2004-01-01
期刊：
Reviews in Environmental Science and Bio/Technology
影响因子：
--
作者：
Field, J. A.;Sierra-Alvarez, R.
通讯作者：
Sierra-Alvarez, R.
影响因子：
3.6
作者：
Aranda, C;Godoy, F;Martínez, M
通讯作者：
Martínez, M
影响因子：
5
作者：
Futamata, H;Harayama, S;Watanabe, K
通讯作者：
Watanabe, K