Preparative isolation and purification of B-type fumonisins by using macroporous resin column and high-speed countercurrent chromatography
Preparative isolation and purification of B-type fumonisins by using macroporous resin column and high-speed countercurrent chromatography
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大孔树脂柱和高速逆流色谱制备分离纯化B型伏马毒素
DOI：
10.1080/19440049.2019.1678768
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发表时间：
2019-10
影响因子：
2.9
通讯作者：
Xu Jianhong
中科院分区：
文献类型：
--
作者：
Hu Junqiang;Lv Hui;Hou Mingxuan;Wang Gang;Lee Yin-Won;Shi Jianrong;Gu Zhenxin;Xu Jianhong
B-type fumonisins (FBs) are water-soluble mycotoxins produced by Fusarium species, which are mainly found in maize products and threaten food safety. Toxicological studies and quantitative determinations of fumonisins require large amounts of pure toxins, and their high prices limit progress in FBs research. In this study, we used a macroporous resin column combined with high-speed countercurrent chromatography to separate large quantities of FBs. A fermented rice culture was extracted with 75% methanol. The dynamic adsorption capacity of FBs on XAD-2 resin was 27.5 mg/g resin at 25 degrees C, pH 4.0, and then the FBs were desorbed with 60% methanol. The crude FBs were further purified using a biphasic system consisting of n-heptane/n-butanol/methanol/water (2:4:1:4, v/v/v/v). The method yielded 1.55 g of FB1 and 0.55 g of FB3 with purities of 96.8% and 95.6%, respectively, from 1 kg of rice culture, and the final overall yield of FBs was 74.8%.
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影响因子：
4.2
作者：
Park J;Chang H;Hong S;Kim D;Chung S;Lee C
通讯作者：
Lee C
影响因子：
6.1
作者：
F. Meredith;C. Bacon;W. P. Norred;R. Plattner
通讯作者：
F. Meredith;C. Bacon;W. P. Norred;R. Plattner
影响因子：
6.1
作者：
通讯作者：
--
影响因子：
8.8
作者：
Lori L Smith;Kyle A Francis;Joseph T Johnson;C. Gaskill
通讯作者：
Lori L Smith;Kyle A Francis;Joseph T Johnson;C. Gaskill
影响因子：
4.3
作者：
Sulyok, Michael;Krska, Rudolf;Schuhmacher, Rainer
通讯作者：
Schuhmacher, Rainer