Glycine and Melatonin Improve Preimplantation Development of Porcine Oocytes Vitrified at the Germinal Vesicle Stage.
Glycine and Melatonin Improve Preimplantation Development of Porcine Oocytes Vitrified at the Germinal Vesicle Stage.
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甘氨酸和褪黑激素改善生泡阶段玻璃化猪卵母细胞的植入前发育
DOI：
10.3389/fcell.2022.856486
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发表时间：
2022
影响因子：
5.5
通讯作者：
Xu B
中科院分区：
文献类型：
--
作者：
Tang Y;Zhang Y;Liu L;Yang Y;Wang Y;Xu B
与其他低脂卵母细胞相比，高脂猪卵母细胞对冷冻保存极其敏感。在卵母细胞冷冻保存中，玻璃化冷冻的效果优于慢速冷冻，并有望通过最大限度地减少细胞渗透和/或氧化应激来进一步改善。在这项研究中，我们比较了加载猪卵丘-卵母细胞复合物与甘氨酸（有机渗透剂）或甘氨酸加褪黑素（内源性抗氧化剂）在玻璃化冷冻，解冻和随后的成熟，以减轻渗透损伤或渗透和氧化损伤的猪卵母细胞的发育潜力的影响。我们的数据表明，甘氨酸治疗显着增加猪卵母细胞的玻璃化冷冻效率的水平与甘氨酸加褪黑激素治疗观察到的。主要表现在解冻后卵母细胞的存活率、核成熟度、活性氧含量、成熟卵母细胞皮质颗粒移位和凋亡发生率、卵丘细胞ATP水平和糖酵解基因转录水平（卵母细胞质量标志）、卵母细胞受精和囊胚发育等方面。但后者比前者更能提高成熟卵母细胞中ATP含量，使线粒体分布正常。两者合计，我们的研究结果表明，减轻玻璃化冷冻和解冻诱导的渗透和氧化应激可以进一步提高玻璃化冷冻猪卵母细胞在萌发囊泡阶段的发育能力。
Lipid-rich porcine oocytes are extremely sensitive to cryopreservation compared to other low-lipid oocytes. Vitrification has outperformed slowing freezing in oocyte cryopreservation and is expected to improve further by minimizing cellular osmotic and/or oxidative stresses. In this study, we compared the effects of loading porcine cumulus-oocyte complexes with glycine (an organic osmolyte) or glycine plus melatonin (an endogenous antioxidant) during vitrification, thawing and subsequent maturation to mitigate osmotic injuries or osmotic and oxidative damages on the developmental potential of porcine oocytes. Our data demonstrated that glycine treatment significantly increased the vitrification efficiency of porcine oocytes to levels comparable to those observed with glycine plus melatonin treatment. It was manifested as the thawed oocyte viability, oocyte nuclear maturation, contents of reactive oxygen species, translocation of cortical granules and apoptotic occurrence in mature oocytes, levels of ATP and transcripts of glycolytic genes in cumulus cells (markers of oocyte quality), oocyte fertilization and blastocyst development. However, the latter was more likely than the former to increase ATP contents and normal mitochondrial distribution in mature oocytes. Taken together, our results suggest that mitigating osmotic and oxidative stresses induced by vitrification and thawing can further enhance the developmental competency of vitrified porcine oocytes at the germinal vesicle stage.
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影响因子：
3.6
作者：
Hadi, T;Hammer, MA;Baltz, JM
通讯作者：
Baltz, JM
影响因子：
3.6
作者：
Combelles, CMH;Albertini, DF
通讯作者：
Albertini, DF
影响因子：
4.1
作者：
Chen, SU;Lien, YR;Lee, TY
通讯作者：
Lee, TY
影响因子：
2.7
作者：
Isachenko, V;Soler, C;Grishchenko, V
通讯作者：
Grishchenko, V
影响因子：
3.4
作者：
Casillas F;Betancourt M;Cuello C;Ducolomb Y;López A;Juárez-Rojas L;Retana-Márquez S
通讯作者：
Retana-Márquez S